Lemoni Zafeiria, Kalantzi Styliani, Lymperopoulou Theopisti, Tzani Andromachi, Stavropoulos Georgios, Detsi Anastasia, Mamma Diomi
Biotechnology Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 9 Iroon Polytechniou Str, 15780 Athens, Greece.
Products and Operations Quality Control Laboratory, School of Chemical Engineering, Zografou Campus, National Technical University of Athens, 15780 Athens, Greece.
Antioxidants (Basel). 2025 May 7;14(5):558. doi: 10.3390/antiox14050558.
This study investigates the enzyme-assisted extraction (EAE) of bioactive compounds from the pseudo-fruit of the wild rose ( L.), also known as rosehip, using a commercial cellulolytic enzyme preparation, Cellic CTec3 HS. The effects of extraction time, solid to liquid ratio, and enzyme loading on total phenolic content (TPC) and total flavonoid content (TFC) were evaluated. The highest yields of TPC (168.3 ± 1.1 GAE/g DM) and TFC (72.3 ± 0.8 mg CAE/g DM) were obtained at 360 min, using 1% (/) enzyme loading and a 6% (/) solid to liquid ratio. Kinetic modeling of the extraction process was performed using first-order, second-order, Peleg's, and power law models. The power law model best described the extraction dynamics. The obtained extracts were assessed for their biological activities including antioxidant, antimicrobial, anti-aging, and anti-diabetic properties. The extract obtained under optimal extraction conditions exhibited potent tyrosinase inhibition (80%) and moderate to low inhibition of α-glucosidase (15%) and α-amylase (20%) activities. The IC for DPPH radical scavenging was 0.44 μL extract/mL while the extract exhibited significant antibacterial activity against growth (79% inhibition). These findings suggest that the extract, obtained through EAE, has promising biological properties with potential applications in the food, pharmaceutical, and cosmetic industries.
本研究使用商业纤维素酶制剂Cellic CTec3 HS，对从野蔷薇（L.）的假果（也称为玫瑰果）中酶辅助提取生物活性化合物进行了研究。评估了提取时间、固液比和酶负载量对总酚含量（TPC）和总黄酮含量（TFC）的影响。在360分钟时，使用1%（/）的酶负载量和6%（/）的固液比，获得了最高的TPC产量（168.3±1.1 GAE/g DM）和TFC产量（72.3±0.8 mg CAE/g DM）。使用一级、二级、佩莱格和幂律模型对提取过程进行动力学建模。幂律模型最能描述提取动力学。对获得的提取物进行了生物活性评估，包括抗氧化、抗菌、抗老化和抗糖尿病特性。在最佳提取条件下获得的提取物表现出强效的酪氨酸酶抑制作用（80%），对α-葡萄糖苷酶（15%）和α-淀粉酶（20%）活性的抑制作用为中度至低度。DPPH自由基清除的IC为0.44 μL提取物/mL，而该提取物对生长表现出显著的抗菌活性（79%抑制）。这些发现表明，通过酶辅助提取获得的提取物具有有前景的生物学特性，在食品、制药和化妆品行业具有潜在应用。
