Truitt K E, Larrick J W, Raubitschek A A, Buck D W, Jacobson S W
Hybridoma. 1984 Summer;3(2):195-9. doi: 10.1089/hyb.1984.3.195.
Human B-cell hybridomas were grown in nude mouse ascites. The growth of cells in ascites requires prior subcutaneous (SC) adaptation, but the amount of antibody harvested is 100-fold that produced in in vitro culture. The relative merits of in vitro versus in vivo production of human monoclonal antibodies are discussed.
