Hugenholtz J, Ivey D M, Ljungdahl L G
Department of Biochemistry, University of Georgia, Athens 30602.
J Bacteriol. 1987 Dec;169(12):5845-7. doi: 10.1128/jb.169.12.5845-5847.1987.
Membrane vesicles of Clostridium thermoautotrophicum prepared by osmotic lysis after lysozyme treatment contained carbon monoxide dehydrogenase and methylenetetrahydrofolate dehydrogenase with specific activities three- to fourfold higher than the specific activity of the cytoplasm. The membrane-associated carbon monoxide dehydrogenase mediated the reduction with CO or the oxidation with CO2 of b-type cytochromes and other electron carriers in the membrane.
经溶菌酶处理后通过渗透裂解制备的嗜热自养梭菌膜囊泡含有一氧化碳脱氢酶和亚甲基四氢叶酸脱氢酶,其比活性比细胞质的比活性高3至4倍。与膜相关的一氧化碳脱氢酶介导了膜中b型细胞色素和其他电子载体被CO还原或被CO₂氧化的过程。