J. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, Gainesville, FL, USA.
Department of Biology & UF Genetics Institute, University of Florida, Gainesville, FL, USA.
Cartilage. 2023 Mar;14(1):94-105. doi: 10.1177/19476035221149146. Epub 2023 Feb 17.
Hyaline cartilage has limited innate healing abilities and hyaline cartilage loss is a hallmark of osteoarthritis (OA). Animal models can provide important insights into cartilage regeneration potential. One such animal model, the African spiny mouse (), is capable of regenerating skin, skeletal muscle, and elastic cartilage. This study aims to evaluate whether these regenerative abilities protect with meniscal injury from OA-related joint damage and behaviors indicative of joint pain and dysfunction.
received destabilization of the medial meniscus (DMM) surgery ( = 11) or a skin incision ( = 10). Gait testing occurred at 4, 6, 8, 10, and 12 weeks after surgery. At endpoint, joints were processed for histology to assess cartilage damage.
Following joint injury, with DMM surgery altered their walking patterns by increasing the percent stance time on the contralateral limb relative to the operated limb, thereby reducing the amount of time the injured limb must bear weight on its own throughout the gait cycle. Histological grading indicated evidence of OA-related joint damage in with DMM surgery; these changes were primarily driven by loss of structural integrity in the hyaline cartilage.
developed gait compensations, and the hyaline cartilage in is not fully protected from OA-related joint damage following meniscal injury, although this damage was less severe than that historically found in C57BL/6 mice with an identical injury. Thus, do not appear to be completely protected from OA-related changes, despite the ability to regenerate other wounded tissues.
透明软骨先天愈合能力有限，透明软骨丧失是骨关节炎（OA）的标志。动物模型可为软骨再生潜力提供重要的见解。非洲刺毛鼠（）是一种这样的动物模型，它能够再生皮肤、骨骼肌和弹性软骨。本研究旨在评估这些再生能力是否能保护非洲刺毛鼠免受半月板损伤引起的 OA 相关关节损伤和关节疼痛及功能障碍的行为。
非洲刺毛鼠（）接受内侧半月板（DMM）手术（=11）或皮肤切口（=10）。手术后 4、6、8、10 和 12 周进行步态测试。在终点时，对关节进行组织学处理以评估软骨损伤。
在关节损伤后，接受 DMM 手术的非洲刺毛鼠改变了它们的行走模式，通过增加对侧肢体相对于手术肢体的站立时间百分比，从而减少了受伤肢体在步态周期中独自承受重量的时间。组织学分级表明 DMM 手术的非洲刺毛鼠存在与 OA 相关的关节损伤；这些变化主要是由透明软骨结构完整性丧失引起的。
尽管非洲刺毛鼠能够再生其他受伤组织，但它们并没有完全免受半月板损伤引起的 OA 相关关节损伤的影响，尽管这种损伤比 C57BL/6 小鼠中相同损伤的 OA 相关变化要轻。因此，尽管非洲刺毛鼠能够再生其他受伤组织，但它们似乎并没有完全免受 OA 相关变化的影响。
