State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Science, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Environ Sci (China). 2023 Jul;129:128-138. doi: 10.1016/j.jes.2022.09.001. Epub 2022 Sep 13.
Bacterial infections have become a great threat to public health in recent years. A primary lysozyme is a natural antimicrobial protein; however, its widespread application is limited by its instability. Here, we present a poly (N-isopropylacrylamide) hydrogel inverse opal particle (PHIOP) as a microcarrier of lysozyme to prolong and enhance the efficiency against bacteria. This PHIOP-based lysozyme (PHIOP-Lys) formulation is temperature-responsive and exhibits long-term sustained release of lysozyme for up to 16 days. It shows a potent antibacterial effect toward both Escherichia coli and Staphylococcus aureus, which is even higher than that of free lysozyme in solution at the same concentration. PHIOPs-Lys were demonstrated to effectively inhibit bacterial infections and enhance wound healing in a full-thickness skin wound rat model. This study provides a novel pathway for prolonging the enzymatic activity and antibacterial effects of lysozyme.
近年来，细菌感染对公众健康构成了巨大威胁。溶菌酶是一种天然的抗菌蛋白，但由于其不稳定性，其广泛应用受到限制。在这里，我们提出了一种聚（N-异丙基丙烯酰胺）水凝胶反蛋白石颗粒（PHIOP）作为溶菌酶的微载体，以延长和增强其对抗细菌的效率。这种基于 PHIOP 的溶菌酶（PHIOP-Lys）制剂具有温度响应性，并表现出长达 16 天的溶菌酶的长期持续释放。它对大肠杆菌和金黄色葡萄球菌都表现出强大的抗菌作用，甚至比相同浓度的游离溶菌酶在溶液中的作用更强。研究表明，PHIOP-Lys 可有效抑制细菌感染，促进全层皮肤伤口大鼠模型的伤口愈合。本研究为延长溶菌酶的酶活性和抗菌效果提供了一种新的途径。
