Bolhari Behnam, Meraji Naghmeh, Seddighi Rahim, Ebrahimi Negar, Chiniforush Nasim
Department of Endodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran.
Photodiagnosis Photodyn Ther. 2023 Jun;42:103345. doi: 10.1016/j.pdpdt.2023.103345. Epub 2023 Feb 16.
The aim of this study is to measure and compare the amount of apical extrusion of the methylene blue as photosensitizer using Laser-activated irrigation(LAI) techniques (SWEEPS, PIPS) and photodynamic therapy (PDT).
40 single-rooted premolar teeth were selected and their root canals were cleaned and prepared. The photosensitizer, Methylene blue (MB), was used as dye which applied inside the root canals and the access cavities. Then the teeth were randomly allocated to 4 groups. Different techniques (SWEEPS, PIPS, PDT) were used in 3 groups. One group received no activation. Data related to the concentration of the extruded methylene blue was measured using ultraviolet and visible (UV-Vis) absorption spectroscopy. Absorption peak intensity of MB in the wavelength of 668 nm was considered as the concentration index according to Beer Lambert law. Taking into account the normal distribution of this data, one-way ANOVA analysis was used to evaluate the effects of the independent variables on the amount of apical extrusion of the irrigant.
Based on the results of ANOVA analysis, there was no significant difference between the amounts of apical extrusion of MB between different groups (p = 0.628) CONCLUSION: Using SWEEPS and PIPS techniques, despite diffusion of the photosensitizer, didn't lead to any difference in the amount of apical extrusion. Therefore, these LAI techniques can be used safely for root canal irrigation.
本研究旨在测量并比较使用激光激活冲洗（LAI）技术（SWEEPS、PIPS）和光动力疗法（PDT）时，作为光敏剂的亚甲蓝的根尖挤出量。
选取40颗单根前磨牙，对其根管进行清理和预备。将光敏剂亚甲蓝（MB）作为染料应用于根管和髓腔。然后将牙齿随机分为4组。3组分别采用不同技术（SWEEPS、PIPS、PDT）。一组不进行激活处理。使用紫外可见（UV-Vis）吸收光谱法测量与挤出的亚甲蓝浓度相关的数据。根据比尔-朗伯定律，将668nm波长下MB的吸收峰强度作为浓度指标。考虑到该数据呈正态分布，采用单因素方差分析来评估自变量对冲洗液根尖挤出量的影响。
基于方差分析结果，不同组之间MB的根尖挤出量无显著差异（p = 0.628）。结论：使用SWEEPS和PIPS技术时，尽管光敏剂会扩散，但根尖挤出量并无差异。因此，这些LAI技术可安全用于根管冲洗。
