Rostami Golriz, Afrasiabi Shima, Benedicenti Stefano, Signore Antonio, Chiniforush Nasim
Laser Research Center of Dentistry, Dentistry Research Institute, Tehran University of Medical Sciences, Tehran 1441987566, Iran.
Department of Surgical Sciences and Integrated Diagnostics, University of Genoa, Viale Benedetto XV 6, 16132 Genoa, Italy.
Biomedicines. 2023 Jun 28;11(7):1850. doi: 10.3390/biomedicines11071850.
This study aimed to assess the efficacy of shockwave-enhanced emission photoacoustic streaming (SWEEPS) plus antimicrobial photodynamic therapy (aPDT) using indocyanine green (ICG) for the elimination of biofilm from infected root canals.
thirty sound human single-canal teeth were chosen and standardized to have 12 mm of root length. The root canals were shaped and prepared by means of ProTaper rotary files. After sterilization of the teeth, the canals were inoculated with for 2 weeks. The teeth were then randomly divided into six groups (n = five) of control, ICG, ICG + 808 nm diode laser, ICG + SWEEPS, ICG + 808 nm diode laser + SWEEPS, and 5.25% sodium hypochlorite (NaOCl). Following treatment, the number of colony-forming units (CFUs)/mL were calculated for each group. Statistical analysis was carried out using one-way ANOVA. For multiple comparisons, Tukey's test was used as the post hoc test.
NaOCl alone showed the highest efficacy ( < 0.001). The ICG + 808 nm diode laser + SWEEPS group displayed significantly lower amounts of bacteria than either the ICG + 808 nm diode laser or SWEEPS ( < 0.001). There was a statistically significant difference detected between the ICG + 808 nm diode laser and ICG + SWEEPS ( = 0.035).
SWEEPS can effectively increase the photosensitizer distribution in the root canal space, and its application along with irrigants can bring about promising results.
本研究旨在评估冲击波增强发射光声流（SWEEPS）联合使用吲哚菁绿（ICG）的抗菌光动力疗法（aPDT）清除感染根管生物膜的疗效。
选取30颗健康人单根管牙，标准化使其根长为12mm。使用ProTaper旋转锉对根管进行预备和塑形。牙齿消毒后，将根管接种细菌2周。然后将牙齿随机分为六组（n = 5），分别为对照组、ICG组、ICG + 808nm二极管激光组、ICG + SWEEPS组、ICG + 808nm二极管激光 + SWEEPS组和5.25%次氯酸钠（NaOCl）组。治疗后，计算每组每毫升的菌落形成单位（CFU）数量。采用单因素方差分析进行统计分析。对于多重比较，使用Tukey检验作为事后检验。
单独使用NaOCl显示出最高疗效（P < 0.001）。ICG + 808nm二极管激光 + SWEEPS组的细菌数量显著低于ICG + 808nm二极管激光组或SWEEPS组（P < 0.001）。ICG + 808nm二极管激光组和ICG + SWEEPS组之间存在统计学显著差异（P = 0.035）。
SWEEPS可有效增加根管内光敏剂的分布，其与冲洗剂联合应用可带来有前景 的结果。
