Finkelstein D B, Strausberg S
J Biol Chem. 1979 Feb 10;254(3):796-803.
When a mating type cells of Saccharomyces cerevisiae are exposed to the mating pheromone alpha-factor in liquid cultures, there is a time-dependent loss of alpha-factor activity from the culture fluid. This loss of biological activity can be directly correlated with the proteolysis of the pheromone by a mating type cells. The metabolism of alpha-factor by a mating type cells may be measured by using either in vitro 125I-labeled or in vivo 35S-labeled pheromone. Addition of chloroquine to growing cultures of a mating type cells at concentrations which cause no detectable alterations in cell growth produces a potentiation of alpha-factor mediated cell cycle arrest. This potentiation of alpha-factor activity is directly correlated with the inhibition of alpha-factor proteolysis. Thus, while proteolytic digestion of alpha-factor appears to be related to the mechanism whereby a mating type cells "detoxify" alpha-factor and recover from cell cycle arrest, proteolysis of the mating factor is not necessary for alpha-factor mediated cell cycle arrest.
当酿酒酵母的a交配型细胞在液体培养物中暴露于交配信息素α-因子时，培养液中的α-因子活性会随时间而丧失。这种生物活性的丧失与a交配型细胞对信息素的蛋白水解直接相关。a交配型细胞对α-因子的代谢可以通过使用体外125I标记或体内35S标记的信息素来测量。在a交配型细胞的生长培养物中添加氯喹，其浓度不会引起细胞生长的可检测变化，但会增强α-因子介导的细胞周期停滞。α-因子活性的这种增强与α-因子蛋白水解的抑制直接相关。因此，虽然α-因子的蛋白水解消化似乎与a交配型细胞“解毒”α-因子并从细胞周期停滞中恢复的机制有关，但交配因子的蛋白水解对于α-因子介导的细胞周期停滞并非必需。
