Chan R K
J Bacteriol. 1977 May;130(2):766-74. doi: 10.1128/jb.130.2.766-774.1977.
Mating-type a cells of the yeast Saccharomyces cerevisiae that had been specifically arrested in the G1 phase of the cell cycle by alpha factor, an oligopeptide pheromone made by alpha cells, recovered and resumed cell division after a period of inhibition which was dependent on the concentration of alpha factor used. These treated a cells were more resistant to alpha factor than untreated a cells, but lost their resistance upon further cell division. However, cells arrested for 6 h were no more resistant to alpha factor than cells arrested for only 2.5 h. Mating-type a strains could inactivate or remove alpha factor from the culture fluid, but two a sterile (nonmating) mutants and an a/alpha diploid strain could not. These results suggest that a cells have a mechanism, which may involve uptake or inactivation of alpha factor, for recovering from alpha factor arrest. However, the results do not distinguish between a recovery mechanism which is constitutive and one which is induced by alpha factor. The loss of alpha factor activity during recovery appeared to be primarily cell contact mediated, although an extracellular, diffusible inhibitor of alpha factor that is labile or that functions stoichiometrically could not be ruled out.
酿酒酵母的a交配型细胞被α因子（一种由α细胞产生的寡肽信息素）特异性阻滞在细胞周期的G1期后，经过一段取决于所用α因子浓度的抑制期后恢复并重新开始细胞分裂。这些经过处理的a细胞比未处理的a细胞对α因子更具抗性，但在进一步细胞分裂后失去了抗性。然而，阻滞6小时的细胞对α因子的抗性并不比仅阻滞2.5小时的细胞更强。a交配型菌株能够使培养液中的α因子失活或去除，但两个a不育（不交配）突变体和一个a/α二倍体菌株则不能。这些结果表明，a细胞有一种从α因子阻滞中恢复的机制，这可能涉及α因子的摄取或失活。然而，结果并未区分是组成型的恢复机制还是由α因子诱导的恢复机制。恢复过程中α因子活性的丧失似乎主要是由细胞接触介导的，尽管不能排除存在一种不稳定的或按化学计量起作用的细胞外、可扩散的α因子抑制剂。
