Udden M M, Finkelstein D B
J Bacteriol. 1978 Mar;133(3):1501-7. doi: 10.1128/jb.133.3.1501-1507.1978.
Alpha-factor-mediated cell cycle arrest and mating inhibition of a mating-type cells of Saccharomyces cerevisiae have been examined in liquid cultures. Cell cycle arrest may be monitored unambiguously by the appearance of morphologically abnormal cells after administration of alpha factor, whereas mating inhibition is determined by comparing the mating efficiency in the absence or presence of added alpha factor. For both cell cycle arrest and mating inhibition, a dose-dependent response may be observed at limiting concentrations of the pheromone. If cell cycle arrest and mating inhibition require a small number of alpha-factor molecules, one might expect that responsive/nonresponsive cells = K(alpha factor)(N) where N is the order of dependence of cell cycle arrest (or mating inhibition) on alpha-factor concentration. The value of N has been determined to be 0.98 +/- 0.18 (standard error of the mean) for cell cycle arrest and 1.08 +/- 0.32 for mating inhibition. These results support the notion that saturation of a single site by alpha factor is sufficient to cause cell cycle arrest or mating inhibition of a mating-type cells.
在液体培养中研究了α因子介导的酿酒酵母a交配型细胞的细胞周期停滞和交配抑制。通过在施用α因子后出现形态异常的细胞可以明确监测细胞周期停滞，而交配抑制则通过比较添加α因子与否时的交配效率来确定。对于细胞周期停滞和交配抑制，在信息素的极限浓度下均可观察到剂量依赖性反应。如果细胞周期停滞和交配抑制需要少量的α因子分子，那么人们可能会预期响应/无响应细胞=K（α因子）^N，其中N是细胞周期停滞（或交配抑制）对α因子浓度的依赖阶数。对于细胞周期停滞，已确定N值为0.98±0.18（平均值的标准误差），对于交配抑制，N值为1.08±0.32。这些结果支持这样的观点，即α因子对单个位点的饱和足以导致a交配型细胞的细胞周期停滞或交配抑制。
