Yamamoto Daiki, Hongo Hiroshi, Kosaka Takeo, Aoki Natsumi, Oya Mototsugu, Sato Toshinori
Department of Biosciences and Informatics, Faculty of Science and Technology, Keio University, 223-8522, Kanagawa, Japan.
Department of Urology, Keio University School of Medicine, 160-8582, Tokyo, Japan.
Glycoconj J. 2023 Apr;40(2):199-212. doi: 10.1007/s10719-023-10104-z. Epub 2023 Feb 18.
A low-oxygen (hypoxia) tumor microenvironment can facilitate chemotherapy and radiation therapy resistance in tumors and is associated with a poor prognosis. Hypoxia also affects PCa (prostate cancer) phenotype transformation and causes therapeutic resistance. Although O-glycans are known to be involved in the malignancy of various cancers under hypoxia, the expression and function of O-glycans in PCa are not well understood. In this study, the saccharide primer method was employed to analyze O-glycan expression in PCa cells. Results showed that the expression of sTn antigens was increased in PCa cells under hypoxia. Furthermore, it was found that ST6GalNAc1, the sTn antigen synthase gene, was involved in the migration-proliferation dichotomy and drug resistance in PCa cells under hypoxia. The results of this study will contribute to the development of novel diagnostic markers and drug targets for PCa under hypoxia.
低氧（缺氧）肿瘤微环境可促进肿瘤对化疗和放疗的耐药性，并与预后不良相关。缺氧还会影响前列腺癌（PCa）的表型转化并导致治疗耐药性。尽管已知O-聚糖在缺氧情况下参与各种癌症的恶性发展，但O-聚糖在PCa中的表达和功能尚未得到充分了解。在本研究中，采用糖引物法分析PCa细胞中O-聚糖的表达。结果显示，缺氧条件下PCa细胞中sTn抗原的表达增加。此外，还发现sTn抗原合成酶基因ST6GalNAc1参与了缺氧条件下PCa细胞的迁移-增殖二分法和耐药性。本研究结果将有助于开发针对缺氧条件下PCa的新型诊断标志物和药物靶点。
