Department of Thyroid and Breast Surgery, Wuhan Red Cross Hospital, No. 392, Hong Kong Road, Jianghan District, Wuhan, 430015, Hubei, China.
Department of Rehabilitation Medicine, Wuhan Hankou Hospital, Wuhan, 430012, Hubei, China.
Mol Biotechnol. 2023 Nov;65(11):1824-1835. doi: 10.1007/s12033-023-00684-4. Epub 2023 Feb 20.
CircRNAs have become a hotspot in tumor research owing to their high stability and specific functions. We investigated the function of hsa_circ_0137652 in the onset and progression of breast cancer (BC). The expression of circ_0137652, miR-1205, and CCNB1 in BC tissues and cell lines were detected using RT-qPCR and/or western blotting. Dual-luciferase reporter and RNA immunoprecipitation chip assays were used to confirm any potential connections between circ_0137652, miR-1205, and CCNB1. CCK-8 and clone formation assays (CFA) were used to measure the proliferation of BC cells. The Transwell assay was used to investigate the migration of BC cells, and the impact of circ_0137652 on BC tumor formation in vivo was validated using animal experiments. RT-qPCR results showed that circ_0137652 and CCNB1 in breast cancer tissues were notably upregulated in normal tissues, whereas miR-1205 was prominently downregulated. After silencing circ_0137652, the growth and migration of BC cells were reduced. Animal experiments showed that circ_0137652 hampers the tumorigenesis of BC cells in vivo. Additionally, we found that circ_0137652 functions as a sponge for miR-1205. Moreover, the miR-1205 inhibitor notably facilitated cell proliferation and migration and attenuated the action of circ_0137652 knockdown. Furthermore, miR-1205 inhibits BC progression by targeting CCNB1. Circ_0137652 controls the miR-1205/CCNB1 axis to induce increased breast cancer malignancy. Our findings suggest that circ_0137652 may be a novel target for BC therapy.
CircRNAs 因其高稳定性和特定功能已成为肿瘤研究的热点。我们研究了 hsa_circ_0137652 在乳腺癌（BC）发生和发展中的作用。使用 RT-qPCR 和/或 Western blot 检测 BC 组织和细胞系中 circ_0137652、miR-1205 和 CCNB1 的表达。双荧光素酶报告基因和 RNA 免疫沉淀芯片分析用于验证 circ_0137652、miR-1205 和 CCNB1 之间的潜在联系。CCK-8 和克隆形成实验（CFA）用于测量 BC 细胞的增殖。Transwell 实验用于研究 BC 细胞的迁移，通过动物实验验证 circ_0137652 对 BC 肿瘤形成的体内影响。RT-qPCR 结果显示，乳腺癌组织中 circ_0137652 和 CCNB1 的表达明显高于正常组织，而 miR-1205 的表达明显下调。沉默 circ_0137652 后，BC 细胞的生长和迁移能力降低。动物实验表明，circ_0137652 抑制 BC 细胞在体内的肿瘤发生。此外，我们发现 circ_0137652 作为 miR-1205 的海绵体发挥作用。此外，miR-1205 抑制剂显著促进细胞增殖和迁移，并减弱 circ_0137652 敲低的作用。此外，miR-1205 通过靶向 CCNB1 抑制 BC 进展。Circ_0137652 控制 miR-1205/CCNB1 轴诱导乳腺癌恶性程度增加。我们的研究结果表明，circ_0137652 可能是治疗 BC 的一个新靶点。
