Taskin Asli Aras, Moretti Daiana Nerina, Vögtle F Nora, Meisinger Chris
Institute of Biochemistry and Molecular Biology, ZBMZ, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
CIBSS - Centre for Integrative Biological Signalling Studies, University of Freiburg, Freiburg, Germany.
Methods Mol Biol. 2023;2615:41-55. doi: 10.1007/978-1-0716-2922-2_4.
The isolation of organelles devoid of other cellular compartments is crucial for studying organellar proteomes and the localization of newly identified proteins, as well as for assessing specific organellar functions. Here, we describe a protocol for the isolation of crude and highly pure mitochondria from Saccharomyces cerevisiae and provide methods for testing the functional integrity of the isolated organelles.
分离不含其他细胞区室的细胞器对于研究细胞器蛋白质组、新鉴定蛋白质的定位以及评估特定细胞器功能至关重要。在此，我们描述了一种从酿酒酵母中分离粗制和高纯度线粒体的方案，并提供了测试分离细胞器功能完整性的方法。
