Camagna A, Rossetti L, De Pirro R, Di Franco M, Lauro R, Samoggia P, Caprari P, Salvo G
Clinica Medica II, University of Rome, Italy.
J Endocrinol Invest. 1987 Aug;10(4):371-5. doi: 10.1007/BF03348151.
It has been demonstrated that young RBCs (reticulocytes and early mature erythrocytes) possess more insulin receptors than old RBCs (late mature erythrocytes) but it is not yet known whether insulin receptors on young and old RBCs are regulated similarly. In the present investigation insulin receptors on young and old RBCs have, therefore, been studied in five normal male subjects before and after 2 days dexamethasone ingestion (0.5 mg tablet every 6 h) and, in the same subjects, before and 5 h after ingestion of 75 g glucose. The results obtained clearly demonstrate that dexamethasone increases insulin receptor concentration while glucose ingestion increases both insulin receptor affinity and concentration on young RBCs. By contrast, neither stimuli modify insulin receptors on old RBCs. Studies on RBCs are usually performed on the whole RBC population not taking into account this differential responsiveness of receptors on young versus old RBCs; consequently, this phenomenon might be responsible of the fact that some data reported on RBCs are not in agreement with those reported on monocytes or adipocytes and it should be taken into consideration when using RBCs to evaluate insulin receptor regulation.
业已证明，年轻红细胞（网织红细胞和早期成熟红细胞）比衰老红细胞（晚期成熟红细胞）拥有更多的胰岛素受体，但年轻和衰老红细胞上的胰岛素受体是否受到类似调节尚不清楚。因此，在本研究中，对5名正常男性受试者在摄入地塞米松（每6小时服用0.5毫克片剂）2天前后以及在摄入75克葡萄糖前和摄入后5小时，研究了年轻和衰老红细胞上的胰岛素受体。所获得的结果清楚地表明，地塞米松增加胰岛素受体浓度，而摄入葡萄糖则增加年轻红细胞上胰岛素受体的亲和力和浓度。相比之下，两种刺激均未改变衰老红细胞上的胰岛素受体。对红细胞的研究通常是在整个红细胞群体上进行的，而没有考虑到年轻与衰老红细胞上受体的这种差异反应性；因此，这种现象可能是导致一些关于红细胞的报道数据与单核细胞或脂肪细胞的报道数据不一致的原因，并且在使用红细胞评估胰岛素受体调节时应予以考虑。
