Moncada Danny, Rico Maite, Montero Belén, Rodríguez-Llamazares Saddys, Feijoo-Bandín Sandra, Gualillo Oreste, Lago Francisca, Aragón-Herrera Alana, Salavagione Horacio, Pettinelli Natalia, Bouza Rebeca, Farrag Yousof
Universidade da Coruña, Grupo de Polímeros, Departamento de Física y Ciencias de la Tierra, Escuela Politécnica de Ingeniería de Ferrol, C/ Mendizábal, s/n, 15403 Ferrol, A Coruña, Spain.
Centro de Investigación de Polímeros Avanzados, Edificio Laboratorio CIPA, Av. Collao 1202, Concepción, Chile.
Int J Biol Macromol. 2023 Apr 30;235:123777. doi: 10.1016/j.ijbiomac.2023.123777. Epub 2023 Feb 20.
Injectable and biocompatible novel hybrid hydrogels based on physically crosslinked natural biopolymers and green graphene for potential use in tissue engineering are reported. Kappa and iota carrageenan, locust bean gum and gelatin are used as biopolymeric matrix. The effect of green graphene content on the swelling behavior, mechanical properties and biocompatibility of the hybrid hydrogels is investigated. The hybrid hydrogels present a porous network with three-dimensionally interconnected microstructures, with lower pore size than that of the hydrogel without graphene. The addition of graphene into the biopolymeric network improves the stability and the mechanical properties of the hydrogels in phosphate buffer saline solution at 37 °C without noticeable change in the injectability. The mechanical properties of the hybrid hydrogels were enhanced by varying the dosage of graphene between 0.025 and 0.075 w/v%. In this range, the hybrid hydrogels preserve their integrity during mechanical test and recover the initial shape after removing the applied stress. Meanwhile, hybrid hydrogels with graphene content of up to 0.05 w/v% exhibit good biocompatibility for 3T3-L1 fibroblasts; the cells proliferate inside the gel structure and show higher spreading after 48 h. These injectable hybrid hydrogels with graphene have promising future as materials for tissue repair.
报道了一种基于物理交联天然生物聚合物和绿色石墨烯的可注射且具有生物相容性的新型杂化水凝胶，其有望用于组织工程。κ-卡拉胶、ι-卡拉胶、刺槐豆胶和明胶用作生物聚合物基质。研究了绿色石墨烯含量对杂化水凝胶的溶胀行为、力学性能和生物相容性的影响。杂化水凝胶呈现出具有三维互连微观结构的多孔网络，其孔径比不含石墨烯的水凝胶的孔径小。将石墨烯添加到生物聚合物网络中可提高水凝胶在37℃磷酸盐缓冲盐溶液中的稳定性和力学性能，且可注射性无明显变化。通过将石墨烯的用量在0.025至0.075 w/v%之间变化，增强了杂化水凝胶的力学性能。在此范围内，杂化水凝胶在力学测试过程中保持其完整性，并在去除施加的应力后恢复初始形状。同时，石墨烯含量高达0.05 w/v%的杂化水凝胶对3T3-L1成纤维细胞表现出良好的生物相容性；细胞在凝胶结构内增殖，并在48小时后显示出更高的铺展性。这些含石墨烯的可注射杂化水凝胶作为组织修复材料具有广阔的前景。
