Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Carbohydr Polym. 2019 Jun 15;214:234-249. doi: 10.1016/j.carbpol.2019.03.030. Epub 2019 Mar 12.
An injectable nanohybrid hydrogel with robust mechanical properties was developed based on Methacrylate-Kappa-carrageenan (KaMA)-dopamine functionalized graphene oxide (GOPD) for soft tissue engineering. KaMA-GOPD hydrogels revealed shear-thinning behavior and injectability through interaction of active catechol groups of dopamine with other moieties in the structure of hydrogels. In addition, these interactions promoted mechanical properties of hydrogels, depending on the GOPD content. Noticeably, encapsulation of 20 wt.% GOPD significantly enhanced compressive strength (8-folds) and toughness (6-folds) of KaMA. Furthermore, the hybrid hydrogel consisting of 20 wt.% GOPD significantly reduced energy loss from 70% (at KaMA) to about 61%, after a two-cycle compression test, while significantly enhanced recovery of the KaMA structure. Reinforcing the KaMA with 20 wt.% GOPD resulted in enhanced fibroblast proliferation (2.5-times) and spreading (5.7 times) after 5 days of culture. Based on these findings, KaMA-GOPD hydrogel could be used for cell delivery through the injection process and applied as a suitable bio-ink for 3D-bioproiting process.
基于丙烯酰胺-κ-卡拉胶（KaMA）-多巴胺功能化氧化石墨烯（GOPD）开发了一种具有强机械性能的可注射纳米杂化水凝胶，用于软组织工程。KaMA-GOPD 水凝胶通过多巴胺中的活性儿茶酚基团与水凝胶结构中的其他部分相互作用，表现出剪切稀化行为和可注射性。此外，这些相互作用取决于 GOPD 的含量，从而促进了水凝胶的机械性能。值得注意的是，封装 20wt.%GOPD 可显著提高 KaMA 的压缩强度（提高 8 倍）和韧性（提高 6 倍）。此外，由 20wt.%GOPD 组成的杂化水凝胶在经过两次循环压缩测试后，其能量损耗从 KaMA 的 70%（约）降低到 61%，而 KaMA 结构的恢复显著增强。在 KaMA 中加入 20wt.%GOPD 可使成纤维细胞的增殖（提高 2.5 倍）和铺展（提高 5.7 倍）在培养 5 天后增强。基于这些发现，KaMA-GOPD 水凝胶可通过注射过程用于细胞输送，并可作为适用于 3D 生物打印过程的合适生物墨水。
