Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia.
Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560, Ankara, Turkey.
Chemosphere. 2023 May;324:138180. doi: 10.1016/j.chemosphere.2023.138180. Epub 2023 Feb 20.
In this work, Atropine as the anticholinergic drug was measured using the environmentally friendly sensor. In this regard, Self-cultivated Spirulina platensis with electroless silver was employed as a powder amplifier in carbon paste electrode modification. Also, 1-Hexyl-3 methylimidazolium Hexafluorophosphate (HMIM PF) ion liquid as a conductor binder was used in the suggested electrode construction. Atropine determination was investigated by voltammetry methods. According to voltammograms, the electrochemical behavior of atropine depends on pH, and pH 10.0 was used as the optimal condition. Moreover, the diffusion control process for the electro-oxidation of atropine was verified by the scan rate study, so the diffusion coefficient (D∼ 3.0136×10cm/sec) value was computed from the chronoamperometry study. Furthermore, responses of the fabricated sensor were linear in the concentration range from 0.01 to 800 μM, and the lowest detection limit of the Atropine determination was obtained at 5 nM. Moreover, the stability, reproducibility, and selectivity factors of the suggested sensor were confirmed by the results. Finally, the recovery percentages for atropine sulfate ampoule (94.48-101.58), and water (98.01-101.3) approve of the applicability of the proposed sensor to Atropine determination in real samples.
在这项工作中，我们使用环保传感器来测量抗胆碱药物阿托品。在这方面，我们使用无电镀银自养螺旋藻作为粉末放大器来修饰碳糊电极。此外，我们还在建议的电极结构中使用了 1-己基-3-甲基咪唑六氟磷酸盐 (HMIM PF) 离子液体作为导体粘合剂。我们通过伏安法研究了阿托品的测定。根据伏安图，阿托品的电化学行为取决于 pH 值，我们选择 pH 10.0 作为最佳条件。此外，通过扫描速率研究验证了阿托品电氧化的扩散控制过程，因此通过计时安培法研究计算了扩散系数 (D∼ 3.0136×10cm/sec) 值。此外，所制备传感器的响应在 0.01 至 800 μM 的浓度范围内呈线性，并且在 5 nM 时获得了最低的阿托品检测限。此外，我们通过结果证实了建议的传感器的稳定性、重现性和选择性因素。最后，硫酸阿托品安瓿（94.48-101.58）和水（98.01-101.3）的回收率证明了所提出的传感器在实际样品中测定阿托品的适用性。
