Hospital of Stomatology, Sun Yat-sen University, Guangzhou, 510055, People's Republic of China.
Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, 510055, People's Republic of China.
J Nanobiotechnology. 2023 Feb 22;21(1):62. doi: 10.1186/s12951-023-01811-8.
Guided bone regeneration (GBR) is widely used in treating oral bone defects to exclude the influence of non-osteogenic tissue on the bone healing process. The traditional method of GBR with a titanium mesh to treat large-area bone defects is limited by the deficiency of increased trauma and costs to patients. Herein, a bi-layered scaffold for GBR composed of a fiber barrier layer and a self-healing hydrogel repair layer is successfully fabricated. The barrier layer is a fibrous membrane material with specific porosity constructed by electrospinning, while the functional layer is a self-healing hydrogel material formed by multiple dynamic covalent bonds. The system can provide an osteogenic microenvironment by preventing the infiltration of connective tissue to bone defects, maintain the stability of the osteogenic space through the self-healing property, and regulate the release of bioactive substances in the dynamic physical condition, which is beneficial to osteoblast proliferation, differentiation, and bone regeneration. This study focused on exploring the effects of different crosslinkers and bonding methods on the comprehensive properties of hydrogels. and proved that the hybrid scaffold system has good biocompatibility, cell barrier function and can enhance bone regeneration activity. Thereby it could be a promising clinical strategy for bone regeneration.
引导骨再生（GBR）被广泛应用于治疗口腔骨缺损，以排除非成骨组织对骨愈合过程的影响。传统的钛网 GBR 治疗大面积骨缺损的方法受到增加创伤和患者费用的限制。本研究中，成功制备了一种由纤维屏障层和自修复水凝胶修复层组成的双层 GBR 支架。屏障层是一种由静电纺丝构建的具有特定孔隙率的纤维膜材料，而功能层是由多重动态共价键形成的自修复水凝胶材料。该系统可以通过防止结缔组织渗透到骨缺损中来提供成骨微环境，通过自修复特性维持成骨空间的稳定性，并在动态物理条件下调节生物活性物质的释放，这有利于成骨细胞的增殖、分化和骨再生。本研究重点探讨了不同交联剂和键合方法对水凝胶综合性能的影响，并证明了混合支架系统具有良好的生物相容性、细胞屏障功能，并能增强骨再生活性。因此，它可能是一种有前途的骨再生临床策略。
