Institute of Horticultural Crops, Xinjiang Academy of Agricultural Science (Key Laboratory of Horticulture Crop Genomics Research and Genetic Improvement in Xinjiang), Urumqi, Xinjiang, China.
College of Horticulture, Xinjiang Agricultural University, Urumqi, Xinjiang, China.
PeerJ. 2023 Feb 13;11:e14844. doi: 10.7717/peerj.14844. eCollection 2023.
Wild tomato germplasm is a valuable resource for improving biotic and abiotic stresses in tomato breeding. The is widely present in eukaryotes and involved in growth and development as well as stress response, such as cold, salt, drought, and biotic stress. In the present study, we identified 45 genes in three wild species of tomatoes. The phylogenetic relationships, gene localization to chromosomes, gene structure, gene collinearity, protein interactions, and -acting element prediction of all 45 genes (14 in 15 in and 16 in ) were analyzed. The phylogenetic analysis showed that the all HVA22 proteins from the family Solanaceae were divided into three branches. The identified 45 genes were grouped into four subfamilies, which displayed similar number of exons and expanded in a fragmentary replication manner. The distribution of genes on the chromosomes of the three wild tomato species was also highly similar. RNA-seq and qRT-PCR revealed that genes were expressed in different tissues and induced by drought, salt, and phytohormone treatments. These results might be useful for explaining the evolution, expression patterns, and functional divergence of genes in .
野生番茄种质是改良番茄育种中生物和非生物胁迫的宝贵资源。HVA22 广泛存在于真核生物中，参与生长发育以及胁迫响应，如冷、盐、干旱和生物胁迫。在本研究中，我们从三个野生番茄种中鉴定出了 45 个基因。对所有 45 个基因（14 个在 Solanaceae 中的 15 个，16 个在 Solanum pimpinellifolium 中的）进行了系统发育关系、基因定位到染色体、基因结构、基因共线性、蛋白质相互作用和顺式作用元件预测分析。系统发育分析表明，来自茄科的所有 HVA22 蛋白分为三个分支。鉴定出的 45 个基因分为四个亚家族，它们具有相似数量的外显子，并且以片段复制的方式扩增。三个野生番茄种的染色体上基因的分布也高度相似。RNA-seq 和 qRT-PCR 显示，基因在不同组织中表达，并受干旱、盐和植物激素处理的诱导。这些结果可能有助于解释 HVA22 基因在 中的进化、表达模式和功能分化。
