Temerdashev A, Dmitrieva E, Azaryan A, Gashimova E
Department of Analytical Chemistry, Kuban State University, Krasnodar, 350040, Russia.
Heliyon. 2023 Jan 28;9(2):e13260. doi: 10.1016/j.heliyon.2023.e13260. eCollection 2023 Feb.
The presence of some drugs in meat samples can cause threat to human health, therefore, its analysis is highly desirable for food safety purposes. In this work, a solid-phase extraction procedure for the determination of oxprenolol, a non-selective beta-blocker, and such anabolic agents as methandienone and testosterone in beef meat samples has been developed. Extraction conditions were optimized to achieve high sensitivity and accuracy of the results. The procedure was validated using meat samples free from target analytes. As a result, high selectivity and sensitivity were observed with the detection limits between 0.25 and 1.25 ng/g, and the results were not affected by matrix components. The proposed procedure was applied to the analysis of real beef samples purchased in the market, and the results have revealed the presence of contaminated samples. The concentration of oxprenolol in the contaminated sample was 7 ng/g, methandienone content in the sample was 30 ng/g, while testosterone level was 4 ng/g.
肉类样本中某些药物的存在会对人体健康造成威胁，因此，出于食品安全目的，对其进行分析非常必要。在本研究中，开发了一种固相萃取方法，用于测定牛肉样本中的氧烯洛尔（一种非选择性β受体阻滞剂）以及美雄酮和睾酮等合成代谢药物。对萃取条件进行了优化，以实现结果的高灵敏度和准确性。该方法使用不含目标分析物的肉类样本进行了验证。结果显示，该方法具有高选择性和灵敏度，检测限在0.25至1.25 ng/g之间，且结果不受基质成分影响。所提出的方法应用于市场上购买的实际牛肉样本分析，结果显示存在受污染的样本。受污染样本中氧烯洛尔的浓度为7 ng/g，样本中美雄酮含量为30 ng/g，而睾酮水平为4 ng/g。
