Liang Yongyong, Ding Huaming
Department of Critical Medicine, Dushu Lake Hospital Affiliated to Soochow University, Suzhou, China.
Department of Critical Medicine, Wuzhong People's Hospital, Suzhou, China.
Ann Surg Treat Res. 2023 Feb;104(2):90-100. doi: 10.4174/astr.2023.104.2.90. Epub 2023 Jan 31.
Severe acute pancreatitis (SAP) is a life-threatening inflammatory syndrome of the pancreas. This study aimed to analyze the clinical significance of () and () expression alterations in SAP.
This study included 18 SAP patients in Wuzhong People's Hospital from November 2019 to December 2021 and 18 healthy controls. and expression levels were determined by RT-quantitative PCR. Correlations between / and sex, age, etiology, CRP, procalcitonin, AST, LDH, BUN, Acute Physiology and Chronic Health Evaluation II (APACHE II), Ranson score, Bedside Index for Severity in Acute Pancreatitis (BISAP) score, sequential organ failure assessment (SOFA), and modified computed tomography severity index (MCTSI) score were analyzed. Diagnostic values of and in SAP were analyzed using the receiver-operating characteristic curve. The binding of to was analyzed by chromatin immunoprecipitation.
and were downregulated in peripheral blood of SAP patients. and were negatively correlated with CRP, procalcitonin, AST, LDH, BUN, APACHE II score, Ranson score, BISAP score, SOFA score, and MCTSI score. Sensitivity and specificity of level of <0.9650 for SAP diagnosis were 88.89% and 72.22%, respectively. Sensitivity and specificity of level of <0.8950 for SAP diagnosis were 66.67% and 83.33%, respectively. was enriched in the promoter and was positively correlated with .
and were downregulated in peripheral blood of SAP patients and served as candidate biomarkers for SAP diagnosis. bound to the promoter to promote transcription.
重症急性胰腺炎（SAP）是一种危及生命的胰腺炎症综合征。本研究旨在分析（）和（）表达改变在SAP中的临床意义。
本研究纳入了2019年11月至2021年12月在吴忠市人民医院的18例SAP患者和18例健康对照。通过RT-定量PCR测定（）和（）的表达水平。分析（）/（）与性别、年龄、病因、CRP、降钙素原、AST、LDH、BUN、急性生理与慢性健康状况评分系统II（APACHE II）、兰森评分、急性胰腺炎严重程度床边指数（BISAP）评分、序贯器官衰竭评估（SOFA）以及改良计算机断层扫描严重程度指数（MCTSI）评分之间的相关性。使用受试者工作特征曲线分析（）和（）在SAP中的诊断价值。通过染色质免疫沉淀分析（）与（）的结合情况。
SAP患者外周血中（）和（）表达下调。（）和（）与CRP、降钙素原、AST、LDH、BUN、APACHE II评分、兰森评分、BISAP评分、SOFA评分和MCTSI评分呈负相关。（）水平<0.9650对SAP诊断的敏感性和特异性分别为88.89%和72.22%。（）水平<0.8950对SAP诊断的敏感性和特异性分别为66.67%和83.33%。（）在（）启动子区域富集且与（）呈正相关。
SAP患者外周血中（）和（）表达下调，可作为SAP诊断的候选生物标志物。（）与（）启动子结合以促进（）转录。
