广藿香醇对甲型流感病毒早期生命周期阶段的抑制作用。
Inhibitory effects of Patchouli alcohol on the early lifecycle stages of influenza A virus.
作者信息
Fan Yaohua, Zhang Qiong, Zhang Wen, Lai Yanni, Long Haishan, Huang Huiting, Zhan Shaofeng, Liu Xiaohong, Lai Jielan, Zhang Zhongde, Pan Pan, Su Ziren, Li Geng
机构信息
Laboratory Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen, China.
出版信息
Front Microbiol. 2023 Feb 1;13:938868. doi: 10.3389/fmicb.2022.938868. eCollection 2022.
BACKGROUND
The antiviral activity and underlying mechanism of Patchouli alcohol remain unclear.
METHODS
This study evaluated the cytotoxicity, optimal methods for drug administration, anti-influenza A activity of Patchouli alcohol. The antiviral mechanism of Patchouli alcohol was also assessed qRT-PCR, western blot, hemagglutination inhibiting (HAI) assay, and hemolysis inhibiting assay.
RESULTS
Patchouli alcohol was shown to have low cytotoxicity and its strongest antiviral effect was associated with premixed administration. Patchouli alcohol inhibited virus replication during the early lifecycle stages of influenza A virus infection and specifically prevented expression of the viral proteins, HA and NP. In both the HAI and hemolysis inhibiting assays, Patchouli alcohol was able to block HA2-mediated membrane fusion under low pH conditions. Patchouli alcohol had lower binding energy with HA2 than HA1.
CONCLUSION
These findings suggest that Patchouli alcohol could be a promising membrane fusion inhibitor for the treatment of influenza A infection.
背景
广藿香醇的抗病毒活性及其潜在机制尚不清楚。
方法
本研究评估了广藿香醇的细胞毒性、最佳给药方法、抗甲型流感病毒活性。还通过qRT-PCR、蛋白质免疫印迹法、血凝抑制（HAI）试验和溶血抑制试验评估了广藿香醇的抗病毒机制。
结果
广藿香醇显示出低细胞毒性，其最强的抗病毒作用与预混合给药有关。广藿香醇在甲型流感病毒感染的早期生命周期阶段抑制病毒复制，并特异性地阻止病毒蛋白HA和NP的表达。在HAI试验和溶血抑制试验中，广藿香醇都能够在低pH条件下阻断HA2介导的膜融合。广藿香醇与HA2的结合能低于HA1。
结论
这些发现表明，广藿香醇可能是一种有前途的用于治疗甲型流感感染的膜融合抑制剂。
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