Kozłowska-Geller Monika Anna, Lewitowicz Piotr, Węgierek-Ciuk Aneta, Białek Wioletta Adamus, Piechowska Agnieszka, Głuszek Stanisław Zbigniew
Jan Kochanowski University of Kielce, Poland.
Cent Eur J Immunol. 2022;47(3):275-279. doi: 10.5114/ceji.2022.119893. Epub 2022 Oct 4.
The aim of the study was to assess the epidemic situation among veterinarians of the Świętokrzyskie Voivodeship, Poland, in relation to the control group.
The research was divided into 3 stages. Stage I involved the selection of subjects. In stage II, flow cytometry for immunophenotyping was performed and the percentage of the sub-population of CD4 cells and CD8 cells was assessed. Stage III involved collection of nasopharyngeal swab samples in order to determine the canine coronavirus CR-CoV mRNA with the rT-PCR method.
The percentage of the CD4 and CD8 lymphocyte subpopulation in relation to the total lymphocyte population in veterinarians did not differ statistically from the percentage in the control group. The CD4/CD8 ratio in the group of veterinarians was on average 1.93, and 2.04 in the control group. There was no statistically significant difference between the groups, p = 0.591. Canine CR-CoV mRNA was not detected in any of the veterinarians or in the control group.
None of the veterinarians had a significant increase in T lymphocytes, which could be an effective defense against SARS-CoV-2.
本研究的目的是评估波兰什切青省兽医群体相对于对照组的疫情情况。
研究分为三个阶段。第一阶段涉及研究对象的选择。在第二阶段，进行免疫表型分析的流式细胞术，并评估CD4细胞和CD8细胞亚群的百分比。第三阶段涉及采集鼻咽拭子样本，以便用逆转录聚合酶链反应（rT-PCR）方法检测犬冠状病毒CR-CoV的信使核糖核酸（mRNA）。
兽医群体中CD4和CD8淋巴细胞亚群相对于总淋巴细胞群体的百分比与对照组相比，在统计学上没有差异。兽医组的CD4/CD8比值平均为1.93，对照组为2.04。两组之间没有统计学上的显著差异，p = 0.591。在任何兽医或对照组中均未检测到犬CR-CoV mRNA。
没有一名兽医的T淋巴细胞有显著增加，而T淋巴细胞可能是对抗严重急性呼吸综合征冠状病毒2（SARS-CoV-2）的有效防御。
