Tengkun Nie, Dongdong Wang, Xiaohui Ma, Yue Chen, Qin Chen
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, China.
State Key Laboratory of Crop Stress Biology for Arid Areas, College of Food Science and Engineering, Northwest A&F University, Yangling, China.
Front Plant Sci. 2019 May 14;10:603. doi: 10.3389/fpls.2019.00603. eCollection 2019.
The accumulation of secondary metabolites, such as anthocyanins, in cells plays an important role in colored plants. The synthesis and accumulation of anthocyanins are regulated by multiple genes, of which the R2R3-MYB transcription factor gene family plays an important role. Based on the genomic data in the Potato Genome Sequencing Consortium database (PGSC) and the transcriptome data in the SRA, this study used potato as a model plant to comprehensively analyze the plant anthocyanin accumulation process. The results indicated that the most critical step in the synthesis of potato anthocyanins was the formation of -coumaroyl-CoA to enter the flavonoid biosynthetic pathway. The up-regulated expression of the gene and the down-regulated expression of significantly promoted this process. At the same time, the anthocyanins in the potato were gradually synthesized during the process from leaf transport to tubers. New transcripts of and were cloned and named and , respectively, but the functions of these two new transcripts still need further study. In addition, the sequence characteristics of amino acids in the R2-MYB and R3-MYB domains of potato were preliminarily identified. The aims of this study are to identify the crucial major genes that affect anthocyanin biosynthesis through multi-omics joint analysis and to transform quantitative traits into quality traits, which provides a basis and reference for the regulation of plant anthocyanin biosynthesis. Simultaneously, this study provides the basis for improving the anthocyanin content in potato tubers and the cultivation of new potato varieties with high anthocyanin content.
次生代谢产物（如花色苷）在细胞中的积累在有色植物中起着重要作用。花色苷的合成和积累受多个基因调控，其中R2R3-MYB转录因子基因家族发挥着重要作用。基于马铃薯基因组测序联盟数据库（PGSC）中的基因组数据和SRA中的转录组数据，本研究以马铃薯为模式植物，全面分析植物花色苷的积累过程。结果表明，马铃薯花色苷合成中最关键的步骤是香豆酰辅酶A的形成以进入类黄酮生物合成途径。 基因的上调表达和 的下调表达显著促进了这一过程。同时，马铃薯中的花色苷在从叶片运输到块茎的过程中逐渐合成。克隆了 和 的新转录本，分别命名为 和 ，但这两个新转录本的功能仍需进一步研究。此外，初步鉴定了马铃薯R2-MYB和R3-MYB结构域中氨基酸的序列特征。本研究的目的是通过多组学联合分析鉴定影响花色苷生物合成的关键主基因，并将数量性状转化为品质性状，为植物花色苷生物合成的调控提供依据和参考。同时，本研究为提高马铃薯块茎中花色苷含量及培育高花色苷含量的马铃薯新品种提供了依据。
