Burke R K
Department of Obstetrics and Gynecology, University of Massachusetts Medical School, Worcester.
J Reprod Med. 1987 Oct;32(10):743-6.
In a prospective study the author measured the "before" and "after" effects of in vitro washing and capacitation on sperm characteristics in 25 normospermic men using a microcomputerized system for semen analysis. Each fresh semen sample was divided into aliquots and assigned to one of four groups: group I--untreated sperm; group II--sperm capacitated with Ham's F-10 medium enriched with 20% decomplemented human serum (HHS); group III--sperm capacitated with HHS to which had been added 10% peritoneal washings in 5% dextrose and lactated Ringer's injection obtained from women of proven fertility at the time of laparoscopic tubal ligation; and group IV--sperm capacitated with HHS to which had been added 10% peritoneal washings in D5RL obtained from women with endometriosis (American Fertility Society stages I and II) diagnosed at the time of diagnostic laparoscopy. Semen characteristics, including sperm velocity, percent motility and motility index, in group II were all significantly improved over those in group I. The addition of peritoneal washings to the capacitation medium (group III) did not further improve the indices except for percent motility, which was significantly increased over that in group II (P less than .01). The addition of peritoneal washings from women with proven endometriosis to the capacitation medium, however, had a marked and statistically significant (P less than .0001) detrimental effect. These results suggest that the adverse effect of endometriosis on fertility may be biochemically mediated.
在一项前瞻性研究中，作者使用微机化精液分析系统，测量了体外洗涤和获能对25名精子正常男性精子特征的“之前”和“之后”的影响。每个新鲜精液样本被分成若干份，并分配到四组中的一组：第一组——未处理的精子；第二组——用富含20%补体灭活人血清（HHS）的哈姆F-10培养基使精子获能；第三组——在HHS中加入10%来自腹腔镜输卵管结扎术时已证实有生育能力女性的5%葡萄糖乳酸林格注射液腹腔冲洗液，使精子获能；第四组——在HHS中加入10%来自诊断性腹腔镜检查时诊断为子宫内膜异位症（美国生育协会I期和II期）女性的D5RL腹腔冲洗液，使精子获能。第二组的精液特征，包括精子速度、活动率百分比和活动指数，均比第一组有显著改善。在获能培养基中添加腹腔冲洗液（第三组），除活动率百分比外，并未进一步改善各项指标，该指标比第二组显著增加（P<0.01）。然而，在获能培养基中添加已证实患有子宫内膜异位症女性的腹腔冲洗液，却产生了显著且具有统计学意义（P<0.0001）的有害影响。这些结果表明，子宫内膜异位症对生育能力的不利影响可能是由生化介导的。
