Kaur Shivreet, Gill Harsimardeep S, Breiland Matthew, Kolmer James A, Gupta Rajeev, Sehgal Sunish K, Gill Upinder
Department of Plant Pathology, North Dakota State University, Fargo, ND, United States.
Department of Agronomy, Horticulture and Plant Science, South Dakota State University, Brookings, SD, United States.
Front Plant Sci. 2023 Feb 1;14:1090163. doi: 10.3389/fpls.2023.1090163. eCollection 2023.
Leaf rust, caused by () is among the most devastating diseases posing a significant threat to global wheat production. The continuously evolving virulent races in North America calls for exploring new sources of leaf rust resistance. A diversity panel of 365 bread wheat accessions selected from a worldwide population of landraces and cultivars was evaluated at the seedling stage against four races (TDBJQ, TBBGS, MNPSD and, TNBJS). A wide distribution of seedling responses against the four races was observed. Majority of the genotypes displayed a susceptible response with only 28 (9.8%), 59 (13.5%), 45 (12.5%), and 29 (8.1%) wheat accessions exhibiting a highly resistant response to TDBJQ, TBBGS, MNPSD and, TNBJS, respectively. Further, we conducted a high-resolution multi-locus genome-wide association study (GWAS) using a set of 302,524 high-quality single nucleotide polymorphisms (SNPs). The GWAS analysis identified 27 marker-trait associations (MTAs) for leaf rust resistance on different wheat chromosomes of which 20 MTAs were found in the vicinity of known genes, MTAs, or quantitative traits loci (QTLs) identified in previous studies. The remaining seven significant MTAs identified represent genomic regions that harbor potentially novel genes for leaf rust resistance. Furthermore, the candidate gene analysis for the significant MTAs identified various genes of interest that may be involved in disease resistance. The identified resistant lines and SNPs linked to the QTLs in this study will serve as valuable resources in wheat rust resistance breeding programs.
由（）引起的叶锈病是对全球小麦生产构成重大威胁的最具毁灭性的病害之一。北美不断演变的毒性小种要求探索新的叶锈病抗性来源。从全球地方品种和栽培品种群体中选出的365份面包小麦种质组成的多样性群体在苗期针对四个小种（TDBJQ、TBBGS、MNPSD和TNBJS）进行了评估。观察到对这四个小种的苗期反应分布广泛。大多数基因型表现出感病反应，只有28份（9.8%）、59份（13.5%）、45份（12.5%）和29份（8.1%）小麦种质分别对TDBJQ、TBBGS、MNPSD和TNBJS表现出高抗反应。此外，我们使用一组302,524个高质量单核苷酸多态性（SNP）进行了高分辨率多位点全基因组关联研究（GWAS）。GWAS分析在不同小麦染色体上鉴定出27个叶锈病抗性标记-性状关联（MTA），其中20个MTA在先前研究中鉴定出的已知抗性基因、MTA或数量性状位点（QTL）附近被发现。鉴定出的其余7个显著MTA代表了含有潜在新叶锈病抗性基因的基因组区域。此外，对显著MTA的候选基因分析确定了各种可能参与抗病性的感兴趣基因。本研究中鉴定出的抗性品系和与QTL连锁的SNP将作为小麦抗锈病育种计划中的宝贵资源。
