• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

非洲猪瘟病毒p72表位的鉴定及初步应用

Identification of p72 epitopes of African swine fever virus and preliminary application.

作者信息

Miao Chun, Yang Sicheng, Shao Junjun, Zhou Guangqing, Ma Yunyun, Wen Shenghui, Hou Zhuo, Peng Decai, Guo HuiChen, Liu Wei, Chang Huiyun

机构信息

African Swine Fever Regional Laboratory of China (Lanzhou), State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, China.

Animal Science and Technology College, Guangxi University, Nanning, Guangxi, China.

出版信息

Front Microbiol. 2023 Feb 3;14:1126794. doi: 10.3389/fmicb.2023.1126794. eCollection 2023.

DOI:10.3389/fmicb.2023.1126794
PMID:36819042
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9935695/
Abstract

African swine fever virus (ASFV) causes a highly lethal hemorrhagic viral disease (ASF) of pigs that results in serious losses in China and elsewhere. The development of a vaccine and diagnosis technology for ASFV is essential to prevent and control the spread of ASF. The p72 protein of ASFV is highly immunogenic and reactive, and is a dominant antigen in ASF vaccine and diagnostic research. In this study, 17 p72 monoclonal antibodies (mAbs) were generated. Epitope mapping by a series of overlapping peptides expressed in showed that these mAbs recognized a total of seven (1-7) linear B cell epitopes. These mAbs did not show significant neutralizing activity. Epitopes 1 (HKPHQSKPIL), 2 (PVGFEYENKV), 5 (VNGNSLDEYSS), and 7 (GYKHLVGQEV) are novel. Sequence alignment analysis revealed that the identified epitopes were highly conserved among 27 ASFV strains from nine genotypes. Preliminary screening using known positive and negative sera indicated the diagnostic potential of mAb-2B8D7. The results provide new insights into the antigenic regions of ASFV p72 and will inform the diagnosis of ASFV.

摘要

非洲猪瘟病毒（ASFV）可引发猪的一种高致死性出血性病毒病（ASF），在中国及其他地区造成严重损失。开发针对ASFV的疫苗和诊断技术对于预防和控制ASF的传播至关重要。ASFV的p72蛋白具有高度免疫原性和反应活性，是ASF疫苗和诊断研究中的主要抗原。在本研究中，制备了17种p72单克隆抗体（mAb）。通过在[具体表达系统]中表达的一系列重叠肽进行表位定位，结果表明这些mAb总共识别出7个（1 - 7）线性B细胞表位。这些mAb未表现出显著的中和活性。表位1（HKPHQSKPIL）、表位2（PVGFEYENKV）、表位5（VNGNSLDEYSS）和表位7（GYKHLVGQEV）是新发现的。序列比对分析显示，在来自9个基因型的27株ASFV毒株中，所鉴定出的表位高度保守。使用已知的阳性和阴性血清进行初步筛选，表明mAb - 2B8D7具有诊断潜力。这些结果为ASFV p72的抗原区域提供了新的见解，并将为ASFV的诊断提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9b025ae77f08/fmicb-14-1126794-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9a19d443d651/fmicb-14-1126794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9e73e92a3f7f/fmicb-14-1126794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/efc8f3b52de8/fmicb-14-1126794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/e75c815feb98/fmicb-14-1126794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/216f0a041073/fmicb-14-1126794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/057f034c0033/fmicb-14-1126794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/637b40c6d844/fmicb-14-1126794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/0cf07a57d141/fmicb-14-1126794-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9b025ae77f08/fmicb-14-1126794-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9a19d443d651/fmicb-14-1126794-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9e73e92a3f7f/fmicb-14-1126794-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/efc8f3b52de8/fmicb-14-1126794-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/e75c815feb98/fmicb-14-1126794-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/216f0a041073/fmicb-14-1126794-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/057f034c0033/fmicb-14-1126794-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/637b40c6d844/fmicb-14-1126794-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/0cf07a57d141/fmicb-14-1126794-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/930c/9935695/9b025ae77f08/fmicb-14-1126794-g009.jpg

相似文献

1
Identification of p72 epitopes of African swine fever virus and preliminary application.非洲猪瘟病毒p72表位的鉴定及初步应用
Front Microbiol. 2023 Feb 3;14:1126794. doi: 10.3389/fmicb.2023.1126794. eCollection 2023.
2
Development and characterization of monoclonal antibody against the critical loop structure of african swine fever virus P72 protein.抗非洲猪瘟病毒 P72 蛋白关键环结构单克隆抗体的研制与鉴定。
Vet Microbiol. 2023 Aug;283:109776. doi: 10.1016/j.vetmic.2023.109776. Epub 2023 May 30.
3
Epitope mapping and establishment of a blocking ELISA for mAb targeting the p72 protein of African swine fever virus.表位作图和建立针对非洲猪瘟病毒 p72 蛋白的单抗阻断 ELISA
Appl Microbiol Biotechnol. 2024 May 29;108(1):350. doi: 10.1007/s00253-024-13146-x.
4
Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies.非洲猪瘟病毒P72蛋白中被单克隆抗体识别的新型线性表位的鉴定
Front Microbiol. 2022 Nov 2;13:1055820. doi: 10.3389/fmicb.2022.1055820. eCollection 2022.
5
Identification of Linear Epitopes in the C-Terminal Region of ASFV p72 Protein.非洲猪瘟病毒p72蛋白C端区域线性表位的鉴定
Microorganisms. 2023 Nov 23;11(12):2846. doi: 10.3390/microorganisms11122846.
6
Identification of monoclonal antibody targeting epitope on p72 trimeric spike of African swine fever virus.鉴定针对非洲猪瘟病毒 p72 三聚体刺突的单克隆抗体表位。
Virus Genes. 2023 Aug;59(4):582-590. doi: 10.1007/s11262-023-02003-0. Epub 2023 May 16.
7
A novel conserved B-cell epitope in pB602L of African swine fever virus.非洲猪瘟病毒 pB602L 中一个新的保守 B 细胞表位。
Appl Microbiol Biotechnol. 2024 Dec;108(1):78. doi: 10.1007/s00253-023-12921-6. Epub 2024 Jan 9.
8
Identification of two novel B cell epitopes on E184L protein of African swine fever virus using monoclonal antibodies.利用单克隆抗体鉴定非洲猪瘟病毒 E184L 蛋白上的两个新 B 细胞表位。
Virus Res. 2024 Aug;346:199412. doi: 10.1016/j.virusres.2024.199412. Epub 2024 Jun 11.
9
Novel Epitopes Mapping of African Swine Fever Virus CP312R Protein Using Monoclonal Antibodies.利用单克隆抗体对非洲猪瘟病毒 CP312R 蛋白进行新型表位作图。
Viruses. 2023 Feb 17;15(2):557. doi: 10.3390/v15020557.
10
A highly efficient blocking ELISA based on p72 monoclonal antibody for the detection of African swine fever virus antibodies and identification of its linear B cell epitope.基于 p72 单克隆抗体的高效阻断 ELISA 法用于检测非洲猪瘟病毒抗体及其线性 B 细胞表位鉴定。
Int J Biol Macromol. 2024 May;268(Pt 1):131695. doi: 10.1016/j.ijbiomac.2024.131695. Epub 2024 Apr 18.

引用本文的文献

1
Cloning and Expression of a Truncated Form of the p72 Protein of the African Swine Fever Virus (ASFV) for Application in an Efficient Indirect ELISA System.非洲猪瘟病毒（ASFV）p72蛋白截短形式的克隆与表达及其在高效间接酶联免疫吸附测定（ELISA）系统中的应用
Pathogens. 2025 May 29;14(6):542. doi: 10.3390/pathogens14060542.
2
Designing a multi-epitope vaccine against African swine fever virus using immunoinformatics approach.运用免疫信息学方法设计针对非洲猪瘟病毒的多表位疫苗。
Sci Rep. 2025 May 8;15(1):16044. doi: 10.1038/s41598-025-00705-z.
3
Development and efficacy of a novel mRNA cocktail for the delivery of African swine fever virus antigens and induction of immune responses.

本文引用的文献

1
Identification of novel linear epitopes in P72 protein of African swine fever virus recognized by monoclonal antibodies.非洲猪瘟病毒P72蛋白中被单克隆抗体识别的新型线性表位的鉴定
Front Microbiol. 2022 Nov 2;13:1055820. doi: 10.3389/fmicb.2022.1055820. eCollection 2022.
2
Assessment of the Impact of a Toll-like Receptor 2 Agonist Synthetic Lipopeptide on Macrophage Susceptibility and Responses to African Swine Fever Virus Infection.评估 Toll 样受体 2 激动剂合成脂肽对巨噬细胞易感性和对非洲猪瘟病毒感染反应的影响。
Viruses. 2022 Oct 8;14(10):2212. doi: 10.3390/v14102212.
3
African swine fever virus: A re-emerging threat to the swine industry and food security in the Americas.
一种用于递送非洲猪瘟病毒抗原并诱导免疫反应的新型mRNA鸡尾酒的研发与功效
Microbiol Spectr. 2025 Jun 3;13(6):e0290924. doi: 10.1128/spectrum.02909-24. Epub 2025 Apr 29.
4
A Mutant of Africa Swine Fever Virus Protein p72 Enhances Antibody Production and Regulates the Production of Cytokines.非洲猪瘟病毒蛋白p72的一种突变体可增强抗体产生并调节细胞因子的产生。
Viruses. 2025 Jan 30;17(2):194. doi: 10.3390/v17020194.
5
Design of a Multiepitope Pan-Proteomic mRNA Vaccine Construct Against African Swine Fever Virus: A Reverse Vaccinology Approach.一种针对非洲猪瘟病毒的多表位全蛋白质组mRNA疫苗构建体的设计：反向疫苗学方法
Vet Med Int. 2025 Jan 4;2025:2638167. doi: 10.1155/vmi/2638167. eCollection 2025.
6
Multi-epitope vaccines: a promising strategy against viral diseases in swine.多表位疫苗：一种对抗猪病毒性疾病的有前景的策略。
Front Cell Infect Microbiol. 2024 Dec 20;14:1497580. doi: 10.3389/fcimb.2024.1497580. eCollection 2024.
7
Screening and identification of linear B-cell epitopes on structural proteins of African Swine Fever Virus.非洲猪瘟病毒结构蛋白线性B细胞表位的筛选与鉴定
Virus Res. 2024 Dec;350:199465. doi: 10.1016/j.virusres.2024.199465. Epub 2024 Sep 25.
8
Identification and Characterization of a Novel B Cell Epitope of ASFV Virulence Protein B125R Monoclonal Antibody.鉴定和表征 ASFV 毒力蛋白 B125R 的新型 B 细胞表位 单克隆抗体。
Viruses. 2024 Aug 5;16(8):1257. doi: 10.3390/v16081257.
9
Advancement in the Antigenic Epitopes and Vaccine Adjuvants of African Swine Fever Virus.非洲猪瘟病毒抗原表位与疫苗佐剂的研究进展
Pathogens. 2024 Aug 21;13(8):706. doi: 10.3390/pathogens13080706.
10
Specific Monoclonal Antibodies against African Swine Fever Virus Protease pS273R Revealed a Novel and Conserved Antigenic Epitope.特异性单克隆抗体针对非洲猪瘟病毒蛋白酶 pS273R 揭示了一个新的保守抗原表位。
Int J Mol Sci. 2024 Aug 15;25(16):8906. doi: 10.3390/ijms25168906.
非洲猪瘟病毒：对美洲养猪业和粮食安全再度构成的威胁。
Front Microbiol. 2022 Oct 5;13:1011891. doi: 10.3389/fmicb.2022.1011891. eCollection 2022.
4
Sequential Deletions of Interferon Inhibitors MGF110-9L and MGF505-7R Result in Sterile Immunity against the Eurasia Strain of Africa Swine Fever.连续缺失干扰素抑制剂 MGF110-9L 和 MGF505-7R 可导致对欧亚非瘟毒株产生无菌免疫。
J Virol. 2022 Oct 26;96(20):e0119222. doi: 10.1128/jvi.01192-22. Epub 2022 Oct 5.
5
African Swine Fever Virus: A Review.非洲猪瘟病毒：综述
Life (Basel). 2022 Aug 17;12(8):1255. doi: 10.3390/life12081255.
6
Research progress on the proteins involved in African swine fever virus infection and replication.非洲猪瘟病毒感染与复制相关蛋白的研究进展。
Front Immunol. 2022 Jul 22;13:947180. doi: 10.3389/fimmu.2022.947180. eCollection 2022.
7
African swine fever control and prevention: an update on vaccine development.非洲猪瘟防控：疫苗研发进展更新。
Emerg Microbes Infect. 2022 Dec;11(1):2021-2033. doi: 10.1080/22221751.2022.2108342.
8
Combinational Deletions of MGF360-9L and MGF505-7R Attenuated Highly Virulent African Swine Fever Virus and Conferred Protection against Homologous Challenge.组合缺失 MGF360-9L 和 MGF505-7R 减弱了高致病性非洲猪瘟病毒的毒力，并提供了针对同源性挑战的保护。
J Virol. 2022 Jul 27;96(14):e0032922. doi: 10.1128/jvi.00329-22. Epub 2022 Jul 6.
9
Analysis of surveillance and prevention plan for African Swine Fever in Italy in 2020.2020 年意大利非洲猪瘟监测与防控计划分析。
Vet Med Sci. 2022 Jul;8(4):1502-1508. doi: 10.1002/vms3.824. Epub 2022 Jun 8.
10
Development of a Blocking ELISA Kit for Detection of ASFV Antibody Based on a Monoclonal Antibody Against Full-Length p72.基于抗全长 p72 单克隆抗体的阻断 ELISA 试剂盒的研制及其在 ASFV 抗体检测中的应用
J AOAC Int. 2022 Sep 6;105(5):1428-1436. doi: 10.1093/jaoacint/qsac050.