Zhang Shuai, Wang Liyun, Liang Xinjin, Vorstius Jan, Keatch Robert, Corner George, Nabi Ghulam, Davidson Fordyce, Gadd Geoffrey Michael, Zhao Qi
School of Science and Engineering, University of Dundee, Dundee DD1 4HN, United Kingdom.
Geomicrobiology Group, School of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom.
ACS Biomater Sci Eng. 2019 Jun 10;5(6):2804-2814. doi: 10.1021/acsbiomaterials.9b00071. Epub 2019 May 9.
Catheter-associated urinary tract infection (CAUTI) presents a significant health problem worldwide and is associated with increased morbidity and mortality. Herein, a silver-polytetrafluoroethylene (Ag-PTFE) nanocomposite coating for catheters was developed via a facile wet chemistry method. Benefiting from the synergistic effect of Ag and PTFE, the as-prepared Ag-PTFE-coated catheter exhibited enhanced antibacterial and antiadhesive activities against two CAUTI-associated strains: WT F1693 and F1557. Compared to the uncoated commercial silicone catheters and the Ag-coated catheters, the Ag-PTFE-coated catheters were able to reduce bacterial adhesion by up to 60.3% and 55.2%, respectively. The Ag-PTFE-coated catheters also exhibited strong antibiofilm activity, reducing biofilm coverage by up to 97.4% compared with the commercial silicone catheters. In an in vitro bladder model, the Ag-PTFE-coated catheter displayed excellent anti-infection efficacy against bacteriuria, extending the lifetime of silicone catheters from a mean of 6 days to over 40 days. The Ag-PTFE coating also showed good biocompatibility with fibroblast cells in culture, making it a prospective strategy to overcome current challenges in CAUTI.
导尿管相关尿路感染（CAUTI）在全球范围内是一个重大的健康问题，并且与发病率和死亡率的增加相关。在此，通过一种简便的湿化学方法开发了一种用于导尿管的银-聚四氟乙烯（Ag-PTFE）纳米复合涂层。受益于银和聚四氟乙烯的协同效应，所制备的涂有Ag-PTFE的导尿管对两种与CAUTI相关的菌株：野生型F1693和F1557表现出增强的抗菌和抗粘附活性。与未涂覆的商用硅胶导尿管和涂银导尿管相比，涂有Ag-PTFE的导尿管能够分别将细菌粘附减少多达60.3%和55.2%。涂有Ag-PTFE的导尿管还表现出强大的抗生物膜活性，与商用硅胶导尿管相比，生物膜覆盖率降低多达97.4%。在体外膀胱模型中，涂有Ag-PTFE的导尿管对菌尿显示出优异的抗感染效果，将硅胶导尿管的使用寿命从平均6天延长至40多天。Ag-PTFE涂层在培养中也与成纤维细胞表现出良好的生物相容性，使其成为克服当前CAUTI挑战的一种有前景的策略。
