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在发育后期构建椿象农作物害虫的转录组。

construction of a transcriptome for the stink bug crop pest during late development.

作者信息

Genevcius Bruno C, Torres Tatiana T

机构信息

Department of Genetics and Evolutionary Biology, University of São Paulo, São Paulo, SP, Brazil.

出版信息

GigaByte. 2020 Dec 31;2020:gigabyte11. doi: 10.46471/gigabyte.11. eCollection 2020.

Abstract

is a neotropical stink bug of economic importance for various crops. Little is known about the development of the species, or the genetic mechanisms that may favor the establishment of populations in cultivated plants. Here, we conduct the first large-scale molecular study of . Using tissues derived from the genitalia and the rest of the body for two immature stages of both males and females, we generated RNA-seq data, then assembled and functionally annotated a transcriptome. The -assembled transcriptome contained around 400,000 contigs, with an average length of 688 bp. After pruning duplicated sequences and conducting a functional annotation, the final annotated transcriptome comprised 39,478 transcripts, of which 12,665 were assigned to Gene Ontology (GO) terms. These novel datasets will be invaluable for the discovery of molecular processes related to morphogenesis and immature biology. We hope to contribute to the growing body of research on stink bug evolution and development, as well as to the development of biorational pest management solutions.

摘要

是一种对多种作物具有经济重要性的新热带区椿象。对于该物种的发育,以及可能有利于其在栽培植物上建立种群的遗传机制,人们了解甚少。在此,我们对……进行了首次大规模分子研究。我们使用来自雄性和雌性两个未成熟阶段的生殖器及身体其他部位的组织,生成了RNA测序数据,然后组装并对转录组进行了功能注释。组装后的……转录组包含约40万个重叠群,平均长度为688碱基对。在去除重复序列并进行功能注释后,最终注释的转录组由39478个转录本组成,其中12665个被分配到基因本体论(GO)术语中。这些新的数据集对于发现与形态发生和未成熟生物学相关的分子过程将具有极高价值。我们希望为关于椿象进化与发育的不断增长的研究做出贡献,以及为开发生物合理的害虫管理解决方案做出贡献。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4f0d/9631951/6969fc71827d/gigabyte-2020-11-g001.jpg

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