One-pot bioconversion of fungal lipid to mycodiesel: a sustainable approach.

The conversion of filamentous fungus-based feedstock into Biodiesel holds potential as a sustainable and eco-conscious method for producing alternative liquid fuels. This study examined the comparison of individual Fatty Acid Methyl Esters (FAME) of Aspergillus niger and Curvularia lunata with the consortium of both filamentous fungal cocktail Fatty acid methyl esters (cFAME), following a transesterification process that turned the fungal lipids into myco-based biodiesel productions. cFAME weighs 23.89 g and accumulates to 20.43 g of lipid yield, with 86% of cellular lipids; in contrast, A. niger weighs 12.65 g and pile up 9.5 g of lipid yield, with 75% of cellular lipid, also C. lunata exhibits 8.35 g of dry weight with 4.89 g of lipid concentration, with 59% of cellular lipids. A. niger was known to contain C16-C18 saturated and unsaturated fatty acids possess LAME (C18:2), OAFA (C18:1), and PAME (C16:0) were shown in high percentages accounted for 86.6% in A. niger. The results showed that PUFA was predominant over MUFA and SFA. C. lunata chiefly produces C16 and C18 fatty acids, which are considered favorable for combustion properties with oleic acid (C18:1), linoleic acid (C18:2), palmitic acid (C16:0), and stearic acid (C18:0), on the comparison. However, the FAME profile of C. lunata occupies only 39.07% of the biodiesel quality. Pentadecanoic acid, palmitic acid, palmitoleic acid, Oleic acid, Linolenic acid, Linoleic acis, and Hexanoic acid with the carbon range of C6:0 - C18:3 were observed in cFAME. Based on the biodiesel yield, cFAME scored 20.55%, whereas A. niger with 11.05 and C.lunata 2.45%, respectively. The presence of methyl esters containing various long-chain fatty acids indicates very effective biodiesel assets, as confirmed by GC-MS analysis, which evidenced ignition efficiency, among others. cFAMEs were impacted by high ignition efficiency with > 4 min. Consortium strategies seize attention in different dimensions and have been confirmed by their upregulation in their fatty acid profiles; in the future, the combination of high lipid holders among the fungal kingdom can be an alternative in myco-based biodiesel production.