Zhang Yu-Lu, Guo Xiao-Jing, Huang Xin, Guo Rong-Jun, Lu Xiao-Hong, Li Shi-Dong, Zhang Hao
Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
College of Plant Protection, Jilin Agricultural University, Changchun 130118, China.
Biology (Basel). 2023 Jan 17;12(2):143. doi: 10.3390/biology12020143.
The root microbiota contributes to the plant's defense against stresses and pathogens. However, the co-association pattern of functional bacteria that improves plant resistance has not been interpreted clearly. Using Illumina high-throughput sequencing technology, the root bacterial community profiles of six cucumber cultivars with different resistance in response to the causative agent of cucumber wilt (CFW), f. sp. (), were analyzed. The principal coordinate analysis indicated that the interactions of the cultivars and pathogens drove the cucumber root bacterial communities ( = 0.001). The resistance-specific differential genera across the cultivars were identified, including in the resistant cultivars, unclassified Enterobacteriaceae in resistant CL11 and JY409, in JY409, in moderately resistant ZN106, and unclassified Rhizobiaceae and in susceptible ZN6. The predominant root bacterium accounted for the relative abundance of up to 28.08-61.55%, but dramatically declined to 9.36% in -inoculated susceptible ZN6. ASV103 and ASV48 of Pseudomonadaceae and ASV162 of Enterobacteriaceae were consistently differential across the cultivars at the phylum, genus, and ASV levels. Using the culture-based method, antagonistic strains of Enterobacteriaceae with a high proportion of 51% were isolated. Furthermore, the bacterial complexes of E318 + Ps213 and spp. C1 + C7 reduced the disease index of CFW by 77.2% and 60.0% in the pot experiment, respectively. This study reveals the co-association of specific root bacteria with host plants and reveals insight into the suppressing mechanism of resistant cultivars against CFW disease by regulating the root microbiota.
根际微生物群有助于植物抵御胁迫和病原体。然而，改善植物抗性的功能细菌的共关联模式尚未得到清晰阐释。利用Illumina高通量测序技术，分析了六个对黄瓜枯萎病病原菌（尖孢镰刀菌黄瓜专化型）具有不同抗性的黄瓜品种的根际细菌群落概况。主坐标分析表明，品种与病原体的相互作用驱动了黄瓜根际细菌群落（P = 0.001）。鉴定出了不同品种间具有抗性特异性的差异属，包括抗性品种中的[具体属名未给出]，抗性品种CL11和JY409中的未分类肠杆菌科，JY409中的[具体属名未给出]，中抗品种ZN106中的[具体属名未给出]，以及感病品种ZN6中的未分类根瘤菌科和[具体属名未给出]。优势根际细菌[具体细菌名称未给出]占相对丰度高达28.08 - 61.55%，但在接种病原菌的感病品种ZN6中急剧下降至9.36%。假单胞菌科的ASV103和ASV48以及肠杆菌科的ASV162在门、属和ASV水平上在不同品种间始终存在差异。采用基于培养的方法，分离出了比例高达51%的高比例肠杆菌科拮抗菌株。此外，在盆栽试验中，E318 + Ps213和[具体菌种未给出] spp. C1 + C7的细菌复合物分别使黄瓜枯萎病的病情指数降低了77.2%和60.0%。本研究揭示了特定根际细菌与寄主植物的共关联，并通过调节根际微生物群揭示了抗性品种对黄瓜枯萎病的抑制机制。
