Cui Ketong, Li Peiyong, Huang Jiaqi, Lin Fang, Li Ruibo, Cao Dingguo, Hao Guijuan, Sun Shuhong
Department of Preventive Veterinary Medicine, College of Animal Science and Technology, Shandong Agricultural University, Tai'an 271018, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an 271018, China.
Biology (Basel). 2023 Feb 14;12(2):312. doi: 10.3390/biology12020312.
Phage therapy is widely being reconsidered as an alternative to antibiotics for the treatment of multidrug-resistant bacterial infections, including salmonellosis caused by . As facultative intracellular parasites, could spread by vertical transmission and pose a great threat to both human and animal health; however, whether phage treatment might provide an optional strategy for controlling bacterial vertical infection remains unknown. Herein, we explored the effect of phage therapy on controlling the vertical transmission of serovar Gallinarum biovar Pullorum ( Pullorum), a poultry pathogen that causes economic losses worldwide due to high mortality and morbidity. A phage CKT1 with lysis ability against several serovars was isolated and showed that it could inhibit the proliferation of Pullorum in vitro efficiently. We then evaluated the effect of phage CKT1 on controlling the vertical transmission of Pullorum in an adult broiler breeder model. The results demonstrated that phage CKT1 significantly alleviated hepatic injury and decreased bacterial load in the liver, spleen, heart, ovary, and oviduct of hens, implying that phage CKT1 played an active role in the elimination of colonization in adult chickens. Additionally, phage CKT1 enabled a reduction in the -specific IgG level in the serum of infected chickens. More importantly, the decrease in the Pullorum load on eggshells and in liquid whole eggs revealed that phage CKT1 effectively controlled the vertical transmission of Pullorum from hens to laid eggs, indicating the potential ability of phages to control bacterial vertical transmission.
噬菌体疗法正被广泛重新视为治疗多重耐药细菌感染（包括由……引起的沙门氏菌病）的抗生素替代方法。作为兼性细胞内寄生虫，……可通过垂直传播扩散，对人类和动物健康都构成巨大威胁；然而，噬菌体治疗是否可能为控制细菌垂直感染提供一种可选策略仍不清楚。在此，我们探讨了噬菌体疗法对控制鸡白痢沙门氏菌（鸡伤寒沙门氏菌鸡白痢变种）垂直传播的影响，鸡白痢沙门氏菌是一种家禽病原体，因其高死亡率和发病率在全球造成经济损失。分离出一种对几种……血清型具有裂解能力的噬菌体CKT1，结果表明它能在体外有效抑制鸡白痢沙门氏菌的增殖。然后我们在成年肉种鸡模型中评估了噬菌体CKT1对控制鸡白痢沙门氏菌垂直传播的效果。结果表明，噬菌体CKT1显著减轻了母鸡肝脏损伤，并降低了肝脏、脾脏、心脏、卵巢和输卵管中的细菌载量，这意味着噬菌体CKT1在消除成年鸡体内的……定植方面发挥了积极作用。此外，噬菌体CKT1使感染鸡血清中……特异性IgG水平降低。更重要的是，蛋壳和全蛋液中鸡白痢沙门氏菌载量的降低表明，噬菌体CKT1有效控制了鸡白痢沙门氏菌从母鸡到所产鸡蛋的垂直传播，表明噬菌体具有控制细菌垂直传播的潜在能力。
