Spagnuolo Carmela, Moccia Federica, Tedesco Idolo, Adabbo Eva, Panzella Lucia, Russo Gian Luigi, Napolitano Alessandra
Institute of Food Sciences, National Research Council, I-83100 Avellino, Italy.
Department of Chemical Sciences, University of Naples "Federico II", I-80126 Napoli, Italy.
Antioxidants (Basel). 2023 Feb 9;12(2):421. doi: 10.3390/antiox12020421.
The production of fruit distillates generates solid residues which are potentially rich in bioactive compounds worthy of valorization and exploitation. We report herein the in vitro antioxidant and antiproliferative properties of an extract obtained from the waste of fermented strawberry distillate production. The main low molecular weight phenolic components of the extract were identified as ellagic acid and p-coumaric acid using spectroscopic and chromatographic analysis. The extract exhibited high antioxidant properties, particularly in the ferric reducing/antioxidant power (FRAP) assay, and a high total phenolic content (TPC). It was also able to induce an antiproliferative effect on different human cancer cell lines. A strong decrease in viability in human promyelocytic leukemia (HL-60) cells through a rapid and massive apoptosis were observed. Moreover, at an early time (<30 min), reactive oxygen species (ROS) production and inactivation of the extracellular signal-regulated kinase (ERK)/mitogen-activated protein kinases (MAPK) pathway were detected. Notably, the antiproliferative activity of the sample was comparable to that observed with an analogous extract prepared from unfermented, fresh strawberries. These results bring new opportunities for the valorization of fruit distillery by-products as low-cost resources for the design of bioactive formulations of comparable value to that from fresh food.
水果蒸馏物的生产会产生固体残渣，这些残渣可能富含值得 valorization 和开发的生物活性化合物。我们在此报告了从发酵草莓蒸馏物生产废料中获得的提取物的体外抗氧化和抗增殖特性。通过光谱和色谱分析，提取物的主要低分子量酚类成分被鉴定为鞣花酸和对香豆酸。该提取物表现出高抗氧化特性，特别是在铁还原/抗氧化能力（FRAP）测定中，以及高总酚含量（TPC）。它还能够对不同的人类癌细胞系诱导抗增殖作用。观察到人类早幼粒细胞白血病（HL-60）细胞的活力通过快速大量凋亡而显著降低。此外，在早期（<30分钟），检测到活性氧（ROS）的产生和细胞外信号调节激酶（ERK）/丝裂原活化蛋白激酶（MAPK）途径的失活。值得注意的是，该样品的抗增殖活性与从未发酵的新鲜草莓制备的类似提取物所观察到的活性相当。这些结果为将水果酿酒厂副产品作为低成本资源进行 valorization 带来了新机会，用于设计与新鲜食品具有同等价值的生物活性制剂。
