Riolo Kristian, Rotondo Archimede, La Torre Giovanna Loredana, Marino Ylenia, Franco Gianluca Antonio, Crupi Rosalia, Fusco Roberta, Di Paola Rosanna, Oliva Sabrina, De Marco Giuseppe, Savastano Domenico, Cuzzocrea Salvatore, Gugliandolo Enrico, Giannetto Alessia
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Viale F. Stagno d'Alcontres n. 31, 98166 Messina, Italy.
Department of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98168 Messina, Italy.
Antioxidants (Basel). 2023 Feb 18;12(2):519. doi: 10.3390/antiox12020519.
The black soldier fly (BSF), has been recognized as one of the most promising insect species for its ability to valorize organic waste while producing a valuable larval biomass with a great potential as a sustainable source of nutrients, including proteins and bioactive molecules. In the present study, BSF larvae were used to produce and characterize the protein hydrolysates (BPHs) that were then evaluated for their potential biological activity in vitro. The BPHs obtained from the BSF larvae proteins by enzymatic digestion were characterized by Nuclear Magnetic Resonance (NMR) and polyacrylamide gel electrophoresis and assessed for their antioxidant activity (BPHs in the range of 0.1 to 1.5 mg/mL) in L-929 cells. Our findings show that BPHs can exert a dose-dependent cytoprotective role against HO-iduced oxidative stress in cells. This antioxidant activity relies on the reduction of ROS levels in challenged cells as measured by flow cytometry and fluorescence microscopy, together with the induction and nuclear translocation of Nrf2, as evaluated by qPCR and indirect immunofluorescence analysis, respectively. Overall, our findings on the remarkable biological activity of the BPHs obtained in a large-scale process strongly suggest the application of BPHs as ingredients promoting animal health in feed formulations.
黑水虻因其能够将有机废物转化为有价值的幼虫生物质而被认为是最有前途的昆虫物种之一，这种幼虫生物质具有作为包括蛋白质和生物活性分子在内的可持续营养来源的巨大潜力。在本研究中，黑水虻幼虫被用于生产和表征蛋白质水解物（BPHs），然后对其体外潜在生物活性进行评估。通过酶消化从黑水虻幼虫蛋白质中获得的BPHs通过核磁共振（NMR）和聚丙烯酰胺凝胶电泳进行表征，并在L-929细胞中评估其抗氧化活性（BPHs浓度范围为0.1至1.5mg/mL）。我们的研究结果表明，BPHs可以对细胞中HO诱导的氧化应激发挥剂量依赖性的细胞保护作用。这种抗氧化活性依赖于通过流式细胞术和荧光显微镜测量的受挑战细胞中ROS水平的降低，以及分别通过qPCR和间接免疫荧光分析评估的Nrf2的诱导和核转位。总体而言，我们关于大规模生产过程中获得的BPHs显著生物活性的研究结果强烈表明，BPHs可作为促进饲料配方中动物健康的成分应用。
