Althobaiti Fayez, Abu Ali Ola A, Kamal Islam, Alfaifi Mohammad Y, Shati Ali A, Fayad Eman, Elbehairi Serag Eldin I, Elshaarawy Reda F M, El-Fattah W Abd
Department of Biotechnology, Faculty of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Department of Chemistry, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
Antibiotics (Basel). 2023 Jan 25;12(2):247. doi: 10.3390/antibiotics12020247.
This work reports a new approach for the synthesis of extremely small monodispersed silver nanoparticles (AgNPs) (2.9-1.5) by reduction of silver nitrate in a new series of benzyl alkyl imidazolium ionic liquids (BAIILs)-based microemulsions (3a-f) as media and stabilizing agents. Interestingly, AgNPs isolated from the IILMEs bearing the bulkiest substituents (-butyl and -butyl) (3f) displayed almost no nanoparticle agglomeration. In an in vitro antibacterial test against ESKAPE pathogens, all AgNPs-BAIILs had potent antibiotic activity, as reflected by antibacterial efficiency indices. Furthermore, when compared to other nanoparticles, these were the most effective in preventing biofilm formation by the tested bacterial strains. Moreover, the MTT assay was used to determine the cytotoxicity of novel AgNPs-BAIILs on healthy human skin fibroblast (HSF) cell lines. The MTT assay revealed that novel AgNPs-BAIILs showed no significant toxic effects on the healthy cells. Thus, the novel AgNPs-BAIILs microemulsions could be used as safe antibiotics for skin bacterial infection treatments. AgNPs isolated from BAIIL (3c) was found to be the most effective antibiotic of the nanoparticles examined.
本研究报告了一种新方法，即在一系列新型苄基烷基咪唑鎓离子液体（BAIILs）基微乳液（3a - f）中，通过硝酸银还原反应合成尺寸极小且单分散的银纳米颗粒（AgNPs）（2.9 - 1.5），该微乳液作为介质和稳定剂。有趣的是，从具有最大取代基（-丁基和-丁基）的离子液体微乳液（IILMEs）（3f）中分离出的AgNPs几乎没有纳米颗粒团聚现象。在针对ESKAPE病原体的体外抗菌测试中，所有AgNPs - BAIILs均具有强大的抗菌活性，抗菌效率指数反映了这一点。此外，与其他纳米颗粒相比，这些纳米颗粒在防止受试细菌菌株形成生物膜方面最为有效。此外，采用MTT法测定新型AgNPs - BAIILs对健康人皮肤成纤维细胞（HSF）细胞系的细胞毒性。MTT法显示，新型AgNPs - BAIILs对健康细胞无明显毒性作用。因此，新型AgNPs - BAIILs微乳液可作为治疗皮肤细菌感染的安全抗生素。从BAIIL（3c）中分离出的AgNPs被发现是所检测纳米颗粒中最有效的抗生素。
