Selim Samy, Badr Bahaa M, Moustafa Mohamed H, Saied Ebrahim, Elkady Fathy M, Salama Ayman, Aloufi Abeer S, Algamdi Noura A, Alzahofi Noura N, Abada Emad, Alshammari Mohammed S, Al Jaouni Soad K, Hashem Amr H
Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Jouf University, Sakaka, Saudi Arabia.
Department of Basic and Clinical Medical Sciences, Faculty of Dentistry, Zarqa University, Zarqa, Jordan.
Sci Rep. 2025 Jul 23;15(1):26735. doi: 10.1038/s41598-025-10349-8.
Natural plant extracts provide a cost-effective and eco-friendly option for the synthesis of bimetallic nanoparticles, as opposed to traditional chemical or physical methods. This research involved the bio-fabrication of silver-titanium dioxide bimetallic nanoparticles (Ag-TiO BNPs) utilizing the leaf extract of Pluchea indica. The Ag-TiO BNPs underwent characterization through UV-vis spectroscopy, FTIR, TEM, XRD, and DLS techniques. The UV-Vis spectroscopy results revealed an absorbance peak at 350 nm, which confirms the successful synthesis of Ag-TiO BNPs. TEM observations revealed that the average diameter of the Ag-TiO BNPs varied between 10 and 60 nm. The assessment of the anticancer, antibacterial, and antioxidant bioactivities of the biosynthesized Ag-TiO BNPs was conducted. Results revealed that the IC50 of Ag-TiO BNP against Wi-38 normal cell line was 169.6 µg/mL. Moreover, Ag-TiO BNPs exhibited anticancer activity against MCF-7 cancerous cell line with an IC50 of 33.5 µg/mL. Furthermore, the produced Ag-TiO BNPs exhibited antibacterial properties against a range of pathogenic bacterial strains, with MIC varying from 31.25 to 62.5 µg/mL. Additionally, Ag-TiO BNPs showed antioxidant activity with IC50 225 µg/mL. In conclusion, Ag-TiO BNPs was successfully biosynthesized using P. indica leaves, where it had anticancer, antibacterial, and antioxidant properties.
与传统化学或物理方法相比，天然植物提取物为双金属纳米颗粒的合成提供了一种经济高效且环保的选择。本研究利用印度阔苞菊的叶提取物对银 - 二氧化钛双金属纳米颗粒（Ag-TiO BNPs）进行生物制备。通过紫外可见光谱、傅里叶变换红外光谱、透射电子显微镜、X射线衍射和动态光散射技术对Ag-TiO BNPs进行表征。紫外可见光谱结果显示在350 nm处有一个吸收峰，这证实了Ag-TiO BNPs的成功合成。透射电子显微镜观察表明，Ag-TiO BNPs的平均直径在10至60 nm之间。对生物合成的Ag-TiO BNPs的抗癌、抗菌和抗氧化生物活性进行了评估。结果显示，Ag-TiO BNP对Wi-38正常细胞系的IC50为169.6 μg/mL。此外，Ag-TiO BNPs对MCF-7癌细胞系表现出抗癌活性，IC50为33.5 μg/mL。此外，所制备的Ag-TiO BNPs对一系列致病细菌菌株具有抗菌性能，最低抑菌浓度在31.25至62.5 μg/mL之间变化。此外，Ag-TiO BNPs表现出抗氧化活性，IC50为225 μg/mL。总之，利用印度阔苞菊叶片成功生物合成了Ag-TiO BNPs，其具有抗癌、抗菌和抗氧化特性。
