Cavazana Thamires Priscila, Hosida Thayse Yumi, Sampaio Caio, de Morais Leonardo Antônio, Monteiro Douglas Roberto, Pessan Juliano Pelim, Delbem Alberto Carlos Botazzo
Department of Preventive and Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Rua José Bonifácio, 1193, Araçatuba 16015-050, SP, Brazil.
Postgraduate Program in Health Sciences, University of Western São Paulo (UNOESTE), Rua José Bongiovani, 700-Cidade Universitária, Presidente Prudente 19050-920, SP, Brazil.
Antibiotics (Basel). 2023 Feb 20;12(2):422. doi: 10.3390/antibiotics12020422.
This study evaluated the effects of calcium glycerophosphate (CaGP), with or without fluoride (F), on dual-species biofilms of and . The biofilms were treated three times with 0.125, 0.25, and 0.5% CaGP solutions, with or without 500 ppm F (NaF). Additionally, 500 and 1100 ppm F-solutions and artificial saliva served as controls. After the final treatment, the microbial viability and biofilm structure, metabolic activity, total biomass production, and the composition of the extracellular matrix composition were analyzed. Regardless of the presence of F, 0.25 and 0.5% CaGP promoted a higher biomass production and metabolic activity increase than the controls ( < 0.05). F-free CaGP solutions reduced bacterial cell population significantly more than the 500 ppm F group or the negative control ( < 0.05). All the groups reduced the proteins, and 0.5% CaGP combined with F led to the highest reduction in the carbohydrate and nucleic acids content of the extracellular matrix ( < 0.05). It can be concluded that CaGP alone affected the number of bacterial cells and, when combined with F, reduced its production of biomass, metabolic activity, and the expression of the extracellular matrix components.
本研究评估了含氟（F）或不含氟的甘油磷酸钙（CaGP）对变形链球菌和远缘链球菌双菌种生物膜的影响。生物膜分别用0.125%、0.25%和0.5%的CaGP溶液处理三次，溶液含或不含500 ppm氟（NaF）。此外，500 ppm和1100 ppm的氟溶液以及人工唾液作为对照。在最后一次处理后，分析了微生物活力、生物膜结构、代谢活性、总生物量产生以及细胞外基质成分的组成。无论是否存在氟，0.25%和0.5%的CaGP均比对照组促进了更高的生物量产生和代谢活性增加（P<0.05）。不含氟的CaGP溶液比500 ppm氟组或阴性对照组显著更多地减少了细菌细胞数量（P<0.05）。所有组均降低了蛋白质含量，0.5%的CaGP与氟结合导致细胞外基质中碳水化合物和核酸含量的降低幅度最大（P<0.05）。可以得出结论，单独使用CaGP会影响细菌细胞数量，当与氟结合时，会降低其生物量产生、代谢活性以及细胞外基质成分的表达。
