使用癌细胞模型分析橄榄（L.）叶提取物和纯化合物的抗氧化和抗病毒作用。

Analysis of Antioxidant and Antiviral Effects of Olive ( L.) Leaf Extracts and Pure Compound Using Cancer Cell Model.

机构信息

Department of Chemical, Biological, Pharmaceutical, and Environmental Sciences, University of Messina, Viale SS. Annunziata, 98168 Messina, Italy.

Unit of Biotechnology and Pathologies, Higher Institute of Biotechnology of Sfax, University of Sfax, Sfax 3029, Tunisia.

出版信息

Biomolecules. 2023 Jan 27;13(2):238. doi: 10.3390/biom13020238.

The present study aims to assess the antioxidant and antiviral effectiveness of leaf extracts obtained from L. var. and L. var. . The total antioxidant activity was determined via both an ammonium phosphomolybdate assay and a nitric oxide radical inhibition assay. Both extracts showed reducing abilities in an in vitro system and in human HeLa cells. Indeed, after oxidative stress induction, we found that exposition to olive leaf extracts protects human HeLa cells from lipid peroxidation and increases the concentration of enzyme antioxidants such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase. Additionally, OESA treatment affects viral DNA accumulation more than OESY, probably due to the exclusive oleuropein content. In fact, subtoxic concentrations of oleuropein inhibit HSV-1 replication, stimulating the phosphorylation of PKR, c-FOS, and c-JUN proteins. These results provide new knowledge about the potential health benefits and mechanisms of action of oleuropein and oleuropein-rich extracts.

本研究旨在评估和叶提取物的抗氧化和抗病毒功效。通过磷钼酸铵测定法和一氧化氮自由基抑制测定法测定总抗氧化活性。两种提取物均在体外系统和人宫颈癌细胞系 HeLa 中显示出还原能力。事实上，在诱导氧化应激后，我们发现橄榄叶提取物暴露于人宫颈癌细胞系 HeLa 中可防止脂质过氧化，并增加过氧化氢酶 (CAT)、超氧化物歧化酶 (SOD) 和谷胱甘肽过氧化物酶等酶抗氧化剂的浓度。此外，OESA 处理对病毒 DNA 积累的影响大于 OESY，这可能是由于橄榄苦苷的含量。事实上，橄榄苦苷的亚毒性浓度可抑制 HSV-1 的复制，刺激 PKR、c-FOS 和 c-JUN 蛋白的磷酸化。这些结果提供了关于橄榄苦苷和富含橄榄苦苷的提取物的潜在健康益处和作用机制的新知识。