Pharmactive Biotech Products S.L. Parque Científico de Madrid, Madrid, Spain.
Departamento de Química Física Aplicada, Facultad de Ciencias, CIAL (CEI, CSIC-UAM), 16722Universidad Autónoma de Madrid, Madrid, Spain.
Antivir Chem Chemother. 2021 Jan-Dec;29:20402066211063391. doi: 10.1177/20402066211063391.
Seasonal flu is caused by influenza infection, a virus that spreads easily in human population with periodical epidemic outbreaks. The high mutational rate of influenza viruses leads to the emergence of strains resistant to the current treatments. Due to that, scientific research is focusing on the development of new anti-influenza agents as alternative or complementary treatments. Olive tree ( L.) has been a source of ancestral remedies due to its antimicrobial activity. Thus, the aim of this study was to test the anti-influenza activity of a standardized olive leaf extract rich in elenolic acid (EA), Isenolic, compared with oseltamivir. Isenolic extract was characterized by High Performance Liquid Chromatography (HPLC)-Mass Spectrometry and its content in EA was determined by HPLC. Cytotoxicity, viral neuraminidase inhibitor activity and cell viability protection against influenza infection of Isenolic were tested using sialic acid overexpressing Madin-Darby Canine Kidney cells. Isenolic formulations showed a 4% and 8% dry basis. Oseltamivir and Isenolic extracts showed anti-influenza activity. The 8% Isenolic formulation showed a dose-dependent neuraminidase inhibitor activity higher than the 4% formulation, and preserved cell viability under viral infection. Thus, Isenolic become a promising natural alternative to existing influenza treatments.
季节性流感是由流感病毒感染引起的，这种病毒在人群中很容易传播，并且会定期爆发流行。流感病毒的高突变率导致了对现有治疗方法产生耐药性的菌株的出现。因此，科学研究集中在开发新的抗流感药物作为替代或补充治疗方法。橄榄树（L.）因其具有抗菌活性而成为传统疗法的来源。因此，本研究旨在测试富含橄榄苦苷（EA）、异土木香内酯的标准化橄榄叶提取物的抗流感活性，与奥司他韦进行比较。Isenolic 提取物通过高效液相色谱-质谱法（HPLC-MS）进行了表征，并通过 HPLC 测定了其 EA 的含量。使用过度表达唾液酸的 Madin-Darby Canine Kidney 细胞测试了 Isenolic 的细胞毒性、病毒神经氨酸酶抑制剂活性和对流感感染的细胞活力保护作用。Isenolic 制剂的干基含量为 4%和 8%。奥司他韦和 Isenolic 提取物均显示出抗流感活性。8%的 Isenolic 制剂表现出剂量依赖性的神经氨酸酶抑制剂活性高于 4%的制剂，并且在病毒感染下保持细胞活力。因此，Isenolic 成为现有流感治疗方法的有前途的天然替代品。
