Department of Pharmaceutical Chemistry, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, 41-200 Sosnowiec, Poland.
Cells. 2023 Feb 12;12(4):593. doi: 10.3390/cells12040593.
Flavonoids exert many beneficial properties, such as anticancer activity. They were found to have chemopreventive effects hindering carcinogenesis, and also being able to affect processes important for cancer cell pathophysiology inhibiting its growth or promoting cell death. There are also reports on the chemosensitizing properties of flavonoids, which indicate that they could be used as a support of anticancer therapy. It gives promise for a novel therapeutic approach in tumors characterized by ineffective treatment, such as high-grade gliomas. The research was conducted on the in vitro culture of human SW1783 anaplastic astrocytoma cells incubated with neobavaisoflavone (NEO), doxorubicin, etoposide, and their combinations with NEO. The analyses involved the WST-1 cell viability assay and image cytometry techniques including cell count assay, Annexin V assay, the evaluation of mitochondrial membrane potential, and the cell-cycle phase distribution. We found that NEO affects the activity of doxorubicin and etoposide by reducing the viability of SW1783 cells. The combination of NEO and etoposide caused an increase in the apoptotic and low mitochondrial membrane potential subpopulations of SW1783 cells. Changes in the cell cycle were observed in all combined treatments. These findings indicate a potential chemosensitizing effect exerted by NEO.
类黄酮具有许多有益的特性，如抗癌活性。它们被发现具有化学预防作用，可阻止致癌作用，并且能够影响癌症细胞病理生理学的重要过程，抑制其生长或促进细胞死亡。也有关于类黄酮化学增敏特性的报道，这表明它们可用于支持抗癌治疗。对于治疗效果不佳的肿瘤，如高级别神经胶质瘤，这为一种新的治疗方法提供了希望。本研究在体外培养的人类 SW1783 间变性星形细胞瘤细胞中进行，用新巴西紫檀素（NEO）、阿霉素、依托泊苷及其与 NEO 的组合孵育。分析包括 WST-1 细胞活力测定和图像细胞术技术，包括细胞计数测定、Annexin V 测定、线粒体膜电位评估和细胞周期相分布。我们发现 NEO 通过降低 SW1783 细胞的活力来影响阿霉素和依托泊苷的活性。NEO 和依托泊苷的组合导致 SW1783 细胞凋亡和低线粒体膜电位亚群增加。所有联合处理均观察到细胞周期的变化。这些发现表明 NEO 具有潜在的化学增敏作用。
