ECsens, Department of Analytical Chemistry, Faculty of Sciences, University of Granada, 18071 Granada, Spain.
Unit of Excellence in Chemistry Applied to Biomedicine and the Environment, Faculty of Science, University of Granada, 18071 Granada, Spain.
Biosensors (Basel). 2023 Feb 13;13(2):267. doi: 10.3390/bios13020267.
Here, we propose a microfluidic paper-based analytical device (µPAD) implemented with a near-field communication (NFC) tag as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The proposed method was based on the fact that Ag could oxidize 3,3',5,5'-tetramethylbenzidine (TMB) into oxidized blue TMB. Thus, the presence of GSH could cause the reduction of oxidized TMB, which resulted in a blue color fading. Based on this finding, we developed a method for the colorimetric determination of GSH using a smartphone. A µPAD implemented with the NFC tag allowed the harvesting of energy from a smartphone to activate the LED that allows the capture of a photograph of the µPAD by the smartphone. The integration between electronic interfaces into the hardware of digital image capture served as a means for quantitation. Importantly, this new method shows a low detection limit of 1.0 µM. Therefore, the most important features of this non-enzymatic method are high sensitivity and a simple, fast, portable and low-cost determination of GSH in just 20 min using a colorimetric signal.
在这里，我们提出了一种基于近场通信（NFC）标签的微流控纸基分析器件（µPAD），作为一种便携式、简单和快速的比色法，用于谷胱甘肽（GSH）的测定。该方法基于以下事实：Ag 可以将 3,3',5,5'-四甲基联苯胺（TMB）氧化成氧化的蓝色 TMB。因此，GSH 的存在会导致氧化 TMB 的还原，从而导致蓝色褪色。基于这一发现，我们开发了一种使用智能手机进行比色法测定 GSH 的方法。带有 NFC 标签的 µPAD 可以从智能手机中获取能量来激活 LED，从而允许智能手机拍摄 µPAD 的照片。将电子接口集成到数字图像捕获硬件中是一种定量手段。重要的是，这种新方法的检测限低至 1.0µM。因此，这种非酶方法的最重要特点是灵敏度高，使用比色信号在 20 分钟内即可简单、快速、便携和低成本地测定 GSH。
