Sikora Tomasz, Morawska Karolina, Lisowski Wiesław, Rytel Paweł, Dylong Agnieszka
Military Institute of Chemistry and Radiometry, 00-910 Warsaw, Poland.
Military Institute of Engineer Technology Named after Professor Józef Kosacki, 50-961 Wroclaw, Poland.
Pharmaceuticals (Basel). 2022 Jan 18;15(2):112. doi: 10.3390/ph15020112.
The aim of presented research is to develop a simple and quick method of spectrophotometric detection for the determination of doxorubicin hydrochloride in blood and plasma. Anthracycline antibiotics are among the most effective antineoplastic agents. However, despite their high efficacy in the treatment of various types of cancer, their administration is limited primarily because they exhibit myocardial toxicity. This may be a limiting factor in the dosage of medications; nevertheless, drugs exhibiting this mechanism of action constitute a very important group of chemotherapeutics. One of the more widely studied antibiotics from the anthracycline group is doxorubicin. It exhibits the highest antineoplastic activity from among a number of derivative compounds. Because of the adverse effects of doxorubicin, especially cardiotoxicity, it is important to maintain control of its concentration in body fluids. The method in the study consists of extraction doxorubicin from the plasma or blood and measurements of the absorbance of light in the visible light range in a DOX solution with respect to a reference sample. The research used blood and plasma samples spiked with doxorubicin to give concentrations in the range of 0.2-10 µg/mL. Obtained LODs were 1.6 µg/mL and 1.2 µg/mL, respectively.
本研究的目的是开发一种简单快速的分光光度检测方法，用于测定血液和血浆中的盐酸多柔比星。蒽环类抗生素是最有效的抗肿瘤药物之一。然而，尽管它们在治疗各种类型癌症方面疗效显著，但主要由于其表现出心肌毒性，其应用受到限制。这可能是药物剂量的限制因素；尽管如此，具有这种作用机制的药物构成了非常重要的一类化疗药物。多柔比星是蒽环类抗生素中研究较为广泛的一种。在众多衍生化合物中，它表现出最高的抗肿瘤活性。由于多柔比星的不良反应，尤其是心脏毒性，控制其在体液中的浓度很重要。该研究中的方法包括从血浆或血液中提取多柔比星，并在多柔比星溶液中相对于参考样品测量可见光范围内的吸光度。该研究使用添加了多柔比星的血液和血浆样品，使其浓度范围为0.2 - 10µg/mL。获得的检测限分别为1.6µg/mL和1.2µg/mL。
