Yang X-K, Li P, Zhang C, Leng R-X, Li S, Liu J, Li B-Z, Pan H-F, Ye D-Q
Department of Epidemiology and Biostatistics, School of Public Health, Anhui Medical University, 81 Meishan Road, 230032, Hefei, Anhui, China.
Anhui Provincial Laboratory of Population Health & Major Disease Screening and Diagnosis, Anhui Medical University, Hefei, Anhui, China.
Z Rheumatol. 2017 Sep;76(7):622-629. doi: 10.1007/s00393-016-0169-0.
The IRAK1 and miR-499 polymorphisms play an important role in the etiology of rheumatoid arthritis (RA). Several studies have been carried out to estimate the association between IRAK1 rs3027898 and miR-499 rs3746444 and RA risk; however, the results were inconsistent.
A case control study was carried out to explore the association between IRAK1 rs3027898 and miR-499 rs3746444 and the RA risk in a Chinese population. Meta-analyses combining present with previous studies were conducted to further explore the association.
A total of 386 RA patients were enrolled along with 576 matched healthy controls. Genotyping was performed by using TaqMan genotyping assays on Fluidigm 192.24 system. For the meta-analysis, a systematic literature search was conducted to identify all relevant studies.
This case control study showed that the IRAK1 rs3027898 C allele was associated with increased risk of RA with an odds ratio (OR) = 1.4 and 95 % confidence intervals (CI) = 1.093-1.793, P = 0.008 but miR-499 rs3746444 polymorphisms were not significantly associated with the risk for RA. The meta-analyses included a total of 4 case control studies on IRAK1 rs3027898 and 4 studies on miR-499 rs3746444. The IRAK1 rs3027898 C allele had an overall OR of 1.268 (95 % CI = 1.130-1.424, P < 0.001). After stratification by ethnicity the C allele had an OR of 1.238 (95 % CI = 1.096-1.398, P = 0.001) in Asians. No association between miR-499 rs3746444 polymorphism and RA was found in the overall and Asian populations.
The results from our case control study and the meta-analyses indicate that the IRAK1 rs3027898 C allele is significantly associated with an increased risk of RA, especially in Asians.
白细胞介素 -1 受体相关激酶 1（IRAK1）和微小 RNA -499（miR -499）基因多态性在类风湿关节炎（RA）的病因中起重要作用。已经开展了多项研究来评估 IRAK1 基因 rs3027898 位点和 miR -499 基因 rs3746444 位点与 RA 风险之间的关联；然而，结果并不一致。
开展一项病例对照研究，以探讨 IRAK1 基因 rs3027898 位点和 miR -499 基因 rs3746444 位点与中国人群 RA 风险之间的关联。进行荟萃分析，将本研究与既往研究相结合，以进一步探讨这种关联。
共纳入 386 例 RA 患者和 576 例匹配的健康对照。采用 TaqMan 基因分型检测法在 Fluidigm 192.24 系统上进行基因分型。对于荟萃分析，进行系统的文献检索以识别所有相关研究。
本病例对照研究表明，IRAK1 基因 rs3027898 位点的 C 等位基因与 RA 风险增加相关，优势比（OR）= 1.4，95%置信区间（CI）= 1.093 - 1.793，P = 0.008，但 miR -499 基因 rs3746444 位点的多态性与 RA 风险无显著关联。荟萃分析共纳入 4 项关于 IRAK1 基因 rs3027898 位点的病例对照研究和 4 项关于 miR -499 基因 rs3746444 位点的研究。IRAK1 基因 rs3027898 位点的 C 等位基因总体 OR 为 1.268（95%CI = 1.130 - 1.424，P < 0.001）。按种族分层后，亚洲人群中 C 等位基因的 OR 为 1.238（95%CI = 1.096 - 1.398，P = 0.001）。在总体人群和亚洲人群中均未发现 miR -499 基因 rs3746444 位点多态性与 RA 之间存在关联。
我们的病例对照研究和荟萃分析结果表明，IRAK1 基因 rs3027898 位点的 C 等位基因与 RA 风险增加显著相关，尤其是在亚洲人群中。
