Division of Cell, Developmental and Integrative Biology, School of Medicine, South China University of Technology, Guangzhou 510006, China.
Department of Cell Biology and Genetics, Core Facility of Developmental Biology, Chongqing Medical University, Chongqing 400016, China.
Genes (Basel). 2023 Feb 15;14(2):499. doi: 10.3390/genes14020499.
The multi-level spatial chromatin organization in the nucleus is closely related to chromatin activity. The mechanism of chromatin organization and remodeling attract much attention. Phase separation describes the biomolecular condensation which is the basis for membraneless compartments in cells. Recent research shows that phase separation is a key aspect to drive high-order chromatin structure and remodeling. In addition, chromatin functional compartmentalization in the nucleus which is formed by phase separation also plays an important role in overall chromatin structure. In this review, we summarized the latest work about the role of phase separation in spatial chromatin organization, focusing on direct and indirect effects of phase separation on 3D chromatin organization and its impact on transcription regulation.
核内多层次空间染色质组织与染色质活性密切相关。染色质组织和重塑的机制引起了广泛关注。相分离描述了生物分子凝聚，这是细胞中无膜隔室的基础。最近的研究表明，相分离是驱动高级染色质结构和重塑的关键方面。此外，由相分离形成的核内染色质功能区室化也在整体染色质结构中起着重要作用。在这篇综述中，我们总结了关于相分离在空间染色质组织中的作用的最新工作，重点介绍了相分离对 3D 染色质组织的直接和间接影响及其对转录调控的影响。
