Institute of Sericulture and Tea, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China.
College of Life Sciences, Anqing Normal University, Anqing 246133, China.
Genes (Basel). 2023 Feb 19;14(2):526. doi: 10.3390/genes14020526.
As a valuable Chinese traditional medicinal species, (Sweet) Nakai () is a natural resource with significant economic and ornamental value. However, its genetic information is not well understood. In this study, the complete mitochondrial genome of was assembled and characterized to explore the repeat sequences, recombination events, rearrangements, and IGT, to predict RNA editing sites, and to clarify the phylogenetic and evolutionary relationship. The mitochondrial genome was found to have two circular chromosomes as its major conformation, with a total length of 436,464 bp and 45.2% GC content. The mitochondrial genome contained 54 genes, including 33 unique protein-coding genes, 18 tRNAs, and 3 rRNA genes. Seven pairs of repeat sequences involving recombination events were analyzed. Both the repeat pairs, R1 and R2, played significant roles in mediating the major and minor conformations. In total, 18 MTPTs were identified, 6 of which were complete tRNA genes. There were 454 RNA editing sites in the 33 protein-coding sequences predicted by the PREPACT3 program. A phylogenetic analysis based on 22 species of mitochondrial genomes was constructed and indicated highly conserved PCG sequences. Synteny analyses showed extensive genomic rearrangements in the mitochondrial genome of and closely related species. This work is the first to report the mitochondrial genome, which is of great significance for conducting additional genetic studies on this organism.
作为一种有价值的中国传统药用物种，（甜）Nakai（）是一种具有重要经济和观赏价值的自然资源。然而，其遗传信息尚未得到很好的理解。在这项研究中，我们组装并描述了的完整线粒体基因组，以探索重复序列、重组事件、重排和 IGT，预测 RNA 编辑位点，并阐明其系统发育和进化关系。发现线粒体基因组有两种主要构象的圆形染色体，总长度为 436,464bp，GC 含量为 45.2%。线粒体基因组包含 54 个基因，包括 33 个独特的蛋白质编码基因、18 个 tRNA 和 3 个 rRNA 基因。分析了涉及重组事件的七对重复序列。重复对 R1 和 R2 都在介导主要和次要构象方面发挥了重要作用。总共鉴定出 18 个 MTPT，其中 6 个是完整的 tRNA 基因。通过 PREPACT3 程序预测的 33 个蛋白质编码序列中共有 454 个 RNA 编辑位点。基于 22 种线粒体基因组构建的系统发育分析表明，PCG 序列高度保守。同线性分析显示，在和密切相关物种的线粒体基因组中存在广泛的基因组重排。这项工作首次报道了的线粒体基因组，这对于开展该生物的进一步遗传研究具有重要意义。
