Plant Molecular Phylogenetics Laboratory, Institute of Biology, College of Science, University of the Philippines, Diliman, 1101, Quezon City, Philippines.
Sci Rep. 2024 Mar 1;14(1):5112. doi: 10.1038/s41598-024-55016-6.
Calophyllum soulattri Burm.f. is traditionally used to treat skin infections and reduce rheumatic pain, yet genetic and genomic studies are still limited. Here, we present the first complete mitochondrial genome of C. soulattri. It is 378,262 bp long with 43.97% GC content, containing 55 genes (30 protein-coding, 5 rRNA, and 20 tRNA). Repeat analysis of the mitochondrial genome revealed 194 SSRs, mostly mononucleotides, and 266 pairs of dispersed repeats ( 30 bp) that were predominantly palindromic. There were 23 homologous fragments found between the mitochondrial and plastome genomes. We also predicted 345 C-to-U RNA editing sites from 30 protein-coding genes (PCGs) of the C. soulatrii mitochondrial genome. These RNA editing events created the start codon of nad1 and the stop codon of ccmFc. Most PCGs of the C. soulattri mitochondrial genome underwent negative selection, but atp4 and ccmB experienced positive selection. Phylogenetic analyses showed C. soulattri is a sister taxon of Garcinia mangostana. This study has shed light on C. soulattri's evolution and Malpighiales' phylogeny. As the first complete mitochondrial genome in Calophyllaceae, it can be used as a reference genome for other medicinal plant species within the family for future genetic studies.
藤黄科植物厚叶藤（Calophyllum soulattri Burm.f.）传统上用于治疗皮肤感染和缓解风湿疼痛，但遗传和基因组研究仍然有限。本文首次报道了厚叶藤的完整线粒体基因组。该基因组全长 378,262 bp，GC 含量为 43.97%，包含 55 个基因（30 个蛋白编码基因、5 个 rRNA 和 20 个 tRNA）。线粒体基因组的重复分析显示，有 194 个 SSRs，大多为单核苷酸，266 对散布重复（30 bp）主要为回文序列。线粒体和质体基因组之间存在 23 个同源片段。我们还预测了 30 个蛋白编码基因（PCGs）中的 345 个 C-to-U RNA 编辑位点。这些 RNA 编辑事件创造了 nad1 的起始密码子和 ccmFc 的终止密码子。厚叶藤线粒体基因组的大多数 PCGs 经历了负选择，但 atp4 和 ccmB 经历了正选择。系统发育分析表明，厚叶藤是藤黄属（Garcinia mangostana）的姐妹群。本研究阐明了厚叶藤的进化和五桠果科的系统发育。作为藤黄科的第一个完整线粒体基因组，它可以作为该科其他药用植物物种未来遗传研究的参考基因组。
