Santaló J, Estop A M, Egozcue J
Departament de Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, Spain.
Mutat Res. 1987 Dec;189(4):407-16. doi: 10.1016/0165-1218(87)90050-4.
beta-Propiolactone (beta PL) has been tested on preimplantation mouse embryos for possible genotoxic effects. Tests were performed at different stages of meiosis (late prophase I, diakinesis/metaphase I, anaphase I, telophase I/prophase II and metaphase II) by injecting females at various times after the induction of superovulation. Male and female derived chromosome complements from first-cleavage embryos were analysed before syngamy for cytogenetic abnormalities. A higher proportion of diploid oocytes, produced by the non-extrusion of the first or second polar body, was found after fertilization when the compound was administered immediately before metaphase I or II. No obvious effect was detected at any other time of beta PL exposure. Based on these results, several possible modes of action for beta PL are postulated.
已对β-丙内酯（β-PL）在植入前小鼠胚胎上进行了潜在遗传毒性作用的测试。通过在超排卵诱导后的不同时间给雌性小鼠注射β-PL，在减数分裂的不同阶段（前期I晚期、终变期/中期I、后期I、末期I/前期II和中期II）进行了测试。在配子融合前分析了来自第一次卵裂胚胎的雄性和雌性衍生染色体组型，以检测细胞遗传学异常情况。当在中期I或II之前立即给予该化合物时，受精后发现由第一或第二极体未排出产生的二倍体卵母细胞比例更高。在β-PL暴露的任何其他时间均未检测到明显影响。基于这些结果，推测了β-PL的几种可能作用模式。
