DuFrain R J, Littlefield L G, Wilmer J L
Mutat Res. 1981 Jun;85(3):147-60. doi: 10.1016/0165-1161(81)90030-3.
An appropriate method for evaluating transmissible genetic damage in female germ cells is presented. Analysis of karyotype preparations from preimplantation (6- day) rabbit embryos for consistent structural chromosome abnormalities is described as a sensitive way in which these determinations can be made. A table prepared from summarized research reports shows that less than 1 in 2000 rabbit blastocysts karyotyped thus far has a consistent structural chromosome abnormality. In the new data presented in this study, none of the 90 control blastocysts karyotyped had consistent structural chromosome abnormalities while 13 of 278 from female rabbits treated with streptonigrin (NSC-45383) before conception did. These findings demonstrate that karyotype analysis for consistent chromosome lesions in preimplantation rabbit embryos from females exposed to chemical clastogens can serve as a sensitive and quantitative means of estimating effects on oocytes. The results of the study also show that the consistent structural chromosome lesions in the blastocyst karyotypes are what would be predicted from the mode of action of streptonigrin and the segregation of chromosomes during meiosis. The relative efficiency of this system based on the rabbit as compared to another recently described system bases on the hamster is also discussed.
本文介绍了一种评估雌性生殖细胞中可传播遗传损伤的合适方法。对植入前（6天）兔胚胎的核型制备物进行分析，以检测是否存在一致的染色体结构异常，这被描述为一种进行这些测定的灵敏方法。根据汇总的研究报告编制的表格显示，到目前为止，在接受核型分析的2000个兔囊胚中，只有不到1个存在一致的染色体结构异常。在本研究提供的新数据中，90个对照囊胚进行核型分析后均未发现一致的染色体结构异常，而在受孕前用链黑菌素（NSC - 45383）处理的雌性兔所产生的278个囊胚中，有13个存在这种异常。这些发现表明，对暴露于化学断裂剂的雌性兔植入前胚胎中是否存在一致的染色体损伤进行核型分析，可作为一种灵敏且定量的方法来评估对卵母细胞的影响。研究结果还表明，囊胚核型中一致的染色体结构损伤符合链黑菌素的作用方式以及减数分裂过程中染色体的分离情况。本文还讨论了基于兔的这个系统与另一个最近描述的基于仓鼠的系统相比的相对效率。
