Division of Cardiovascular Physiology, Faculty of Medicine, University of Miyazaki, Miyazaki 8891692, Japan.
School of Biosciences and Technology, Vellore Institute of Technology University, Vellore 632014, India.
Int J Mol Sci. 2023 Feb 6;24(4):3184. doi: 10.3390/ijms24043184.
Currently, there is a great demand for the development of nanomedicine aided wound tissue regeneration via silver doped nanoceuticals. Unfortunately, very little research is being carried out on antioxidants-doped silver nanometals and their interaction on the signaling axis during the bio-interface mechanism. In this study, c-phycocyanin primed silver nano hybrids (AgcPCNP) were prepared and analyzed for properties such as cytotoxicity, metal decay, nanoconjugate stability, size expansion, and antioxidant features. Fluctuations in the expression of marker genes during cell migration phenomena in in vitro wound healing scenarios were also validated. Studies revealed that physiologically relevant ionic solutions did not exhibit any adverse effects on the nanoconjugate stability. However, acidic, alkali, and ethanol solutions completely denatured the AgcPCNP conjugates. Signal transduction RTPCR array demonstrated that genes associated with NFĸB- and PI3K-pathways were significantly ( < 0.5%) altered between AgcPCNP and AgNP groups. Specific inhibitors of NFĸB (Nfi) and PI3K (LY294002) pathways confirmed the involvement of NFĸB signaling axes. In vitro wound healing assay demonstrated that NFĸB pathway plays a prime role in the fibroblast cell migration. In conclusion, the present investigation revealed that surface functionalized AgcPCNP accelerated the fibroblast cell migration and can be further explored for wound healing biomedical applications.
目前，人们对通过掺杂银的纳米药物辅助组织再生的纳米医学有很大的需求。不幸的是，在生物界面机制中，关于抗氧化剂掺杂的银纳米金属及其对信号轴的相互作用的研究很少。在这项研究中，制备了藻蓝蛋白敏化的银纳米杂化物（AgcPCNP），并对其进行了细胞毒性、金属衰减、纳米缀合物稳定性、尺寸扩展和抗氧化特性等性质的分析。还验证了体外伤口愈合情况下细胞迁移现象中标记基因表达的波动。研究表明，生理相关的离子溶液对纳米缀合物的稳定性没有任何不良影响。然而，酸性、碱性和乙醇溶液完全使 AgcPCNP 缀合物变性。信号转导 RTPCR 阵列表明，AgcPCNP 和 AgNP 组之间与 NFĸB 和 PI3K 通路相关的基因显著（<0.5%）改变。NFĸB（Nfi）和 PI3K（LY294002）通路的特异性抑制剂证实了 NFĸB 信号轴的参与。体外伤口愈合试验表明，NFĸB 通路在成纤维细胞迁移中起主要作用。总之，本研究表明，表面功能化的 AgcPCNP 加速了成纤维细胞的迁移，可以进一步探索用于伤口愈合的生物医学应用。
