Department of Clinical Sciences, Swedish University of Agricultural Sciences (SLU), 75007 Uppsala, Sweden.
Biomedical Science and Veterinary Public Health, Swedish University of Agricultural Sciences (SLU), 75007 Uppsala, Sweden.
Int J Mol Sci. 2023 Feb 8;24(4):3435. doi: 10.3390/ijms24043435.
The rapid emergence of antibacterial resistance requires alternatives to antibiotics to be found, including for semen preservation. One of the possible alternatives would be to use plant-based substances with known antimicrobial effects. The objective of this study was to test the antimicrobial effect of pomegranate powder, ginger, and curcumin extract in two concentrations on bull semen microbiota after exposure for <2 h and 24 h. An additional aim was to evaluate the effect of these substances on sperm quality parameters. The bacterial count in semen was low from the beginning; however, a reduction was present for all tested substances compared with control. A reduction in bacterial count in control samples was also observed with time. Curcumin at a concentration of 5%, reduced bacterial count by 32% and was the only substance that had a slight positive effect on sperm kinematics. The other substances were associated with a decline in sperm kinematics and viability. Neither concentration of curcumin had a deleterious effect on sperm viability parameters measured by flow cytometry. The results of this study indicate that curcumin extract at a concentration of 5% can reduce the bacterial count and does not have a negative influence on bull sperm quality.
抗菌耐药性的迅速出现要求找到抗生素的替代品，包括用于精液保存的替代品。一种可能的替代品是使用具有已知抗菌作用的植物性物质。本研究的目的是测试石榴粉、姜和姜黄素提取物在两种浓度下对暴露时间<2 小时和 24 小时后的公牛精液微生物群的抗菌作用。另一个目的是评估这些物质对精子质量参数的影响。从一开始，精液中的细菌计数就很低；然而，与对照组相比，所有测试物质的细菌计数都有所减少。对照样本中的细菌计数也随着时间的推移而减少。浓度为 5%的姜黄素可使细菌计数减少 32%，是唯一对精子运动学有轻微积极影响的物质。其他物质与精子运动学和活力下降有关。两种浓度的姜黄素对通过流式细胞术测量的精子活力参数均没有不良影响。本研究结果表明，浓度为 5%的姜黄素提取物可以降低细菌计数，并且对公牛精子质量没有负面影响。
